双水相萃取丽江山慈菇中的秋水仙碱  

Extraction the Colchicine of Iphigenia indica in Aqueous Two-phase System

作　　者：石瑶[1,2] 杨亚玲[1] 李晚谊[2] 刘谋盛[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明650500 [2]云南省农业科学院药用植物研究所,昆明650223

出　　处：《天然产物研究与开发》2012年第10期1412-1416,共5页Natural Product Research and Development

摘　　要：建立了由聚乙二醇(PEG6000)与(NH4)2SO4形成的双水相体系萃取丽江山慈菇中秋水仙碱的新方法。考察了PEG分子量、PEG的浓度、(NH4)2SO4的浓度和pH值对双水相成相及秋水仙碱萃取率的影响,并结合HPLC对萃取相进行检测。结果表明:PEG6000质量分数为8%,(NH4)2SO4质量分数为20%,pH为7.0时,双水相体系对丽江山慈菇粗提液中秋水仙碱萃取率达82.09%,富集倍数为6.84倍。此方法可用于丽江山慈菇中秋水仙碱的初步分离富集,且操作简单,绿色无污染。A new method to extract the colchicine of lphigenia indica in aqueous two-phase system（ATPS） formed by macromolecular compound PEG6000 and （NH4）2SO4 was investigated. The effect of the molecular weight of PEG, the concentration of PEG, the concentration of （NH4 ）2 SO4 and pH on phase behavior of the ATPS and extraction efficiencies of colchicines was investigated systematically. Determination the content of colchincine in the extraction phase combined with HPLC. The results showed that the best ATPS was composed of 8% PEG6000 and 20% （ NH4 ）2SO4 ,pH 7.0. The extraction efficiency of colchicine of crude extract could reach to 82.09% ,fold enrichment reach to 6.84. This method could be used to initial separate and enrichment the colchicine of Iphigenia indica, simple operation and friendly to the environment.

关 键 词：丽江山慈菇 秋水仙碱 双水相体系 HPLC 

分 类 号：R284.2[医药卫生—中药学]