基于UPLC/Q-TOF-MS不同比例人参配伍藜芦增毒的物质基础及动物毒性关联性研究  

UPLC/Q-TOF-MS Based the Diversity of Toxiferous Composition of the Toxicity of Animals and in Different Combination of Veratrum Nigrum and Ginseng

作　　者：杨亮[1,2] 王宇光[2] 梁乾德[2] 刘浩生[1] 马增春[2] 肖成荣[2] 谭洪玲[2] 汤响林[2] 张伯礼[3] 高月[2] 

机构地区：[1]安徽医科大学研究生学院,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所药理毒理研究室,北京100850 [3]天津中医药大学,天津300193

出　　处：《质谱学报》2012年第5期257-264,共8页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点基础研究发展计划(批准号:2011CB505304;2012CB518402);国家自然科学基金(批准号:81073149);北京市自然科学基金(批准号:7112110)资助

摘　　要：通过小鼠的急性毒性实验,探索中药十八反中藜芦与人参药对不同配比时毒性变化趋势,结合超高效液相色谱-四极杆-飞行时间质谱联用技术(UPLC/Q-TOF-MS)对不同配比对应的毒性成分进行分析,采用MassLynx4.1分析软件,结合主成分分析(PCA)及正交偏最小二乘法判别(OPLS-DA)分析生物碱类成分变化。固定藜芦用量时,藜芦与人参在不同配比情况下小鼠死亡率随人参剂量增大呈下降趋势,藜芦酰棋盘花胺、3-当归酰棋盘花胺、红介芬胺、计米亭碱、藜芦胺和3-藜芦酰基计明碱等生物碱含量在不同配比中的变化趋势与动物毒性变化一致。以上几种生物碱在藜芦与人参合用时可能是毒性效应的关键成分,能够反映配伍毒性的变化趋势。In order to study toxicity changes in di{{erent matching between the herbal gin- seng and veratrum nigrum of eighteen incompatible medication in traditional medicine, the acute toxicity of mice treated with veratrum nigrum-ginseng combination of the eighteen in- compatibal （Shi Ba Fan） was explored. UPLC/TOF-MS with Principal Components Analy- sis （PCA） were used to analyze the same samples used in acute toxic experiment. The re-sults showed that death rate of mice was decreased in the rise of compatibility of ginseng with fixed dose of veratrum nigrum, toxicity alkaloid series such as veratrosine, veratrum alkamine, 3-angeloylzygadenine, germidine, veretramine, rubijervine were also increased. Therefore, above alkaloid series may be key factors contributed to the poisoning effect of ginseng combined with veratrum nigrum.

关 键 词：藜芦 人参 急性毒性 UPLC/Q-TOFMS 

分 类 号：O657.63[理学—分析化学]