独活治疗实验性自身免疫性脑脊髓炎的免疫学机制研究  

Immunological Mechanisms of Duhuo on Experimental Allergic Encephalomyelitis

作　　者：郝海光[1] 李红艳[1] 张晓丹[1] 赵丹[1] 孙东[1] 胡昱[1] 杨静娴[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《时珍国医国药》2012年第10期2407-2410,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81173580);辽宁省自然科学基金(No.21102144);辽宁省沈阳市科技专项资金项目(No.F11-264-1)42

摘　　要：目的探究中药独活对多发性硬化(MS)模型小鼠的治疗作用及机理。方法雌性C57BL/6小鼠,按文献方法制备实验性自身免疫性脑脊髓炎(EAE)模型。将造模小鼠随机分为正常,模型,独活低、中、高剂量,阳性对照药黄芩苷及醋酸泼尼松等7组。各组每日灌胃相应的药物,40天后分别进行EAE神经功能评分、淋巴细胞增殖能力检测、淋巴细胞分泌细胞因子含量测定。结果高剂量独活(36 mg/kg)能明显缓解EAE神经功能受损症状,使临床评分显著降低;能显著抑制淋巴细胞增殖;能使脾淋巴细胞分泌的IFN-γ浓度下降、IL-13浓度升高,上述结果与EAE模型组比较均差异显著(P<0.05,P<0.01)。结论独活能明显减轻EAE神经功能受损症状;显著抑制EAE小鼠脾淋巴细胞的增殖;下调Th1细胞因子IFN-γ、上调Th2细胞因子IL-13,恢复Th1/Th2的平衡,从而有效缓解MS/EAE。Objective To explore the therapeutic effects and immunological mechanisms of traditional Chinese medicine Duhuo on the mice model of multiple sclerosis. Methods Female C57BL/6 mice were used for the establishment of experimental allergic en- cephalomyelitis （EAE） model according to the reports, then randomly divided into seven groups. Drugs were administered p. o once a day for 40 days, and clinical scores, capacity of lymphocyte proliferation and cytokines levels secreted by lymphocyte were detected. Results Duhuo of high dose （36mg/kg） reduced clinical scores of EAE mice and inhibited the proliferation of lympho- cyte obviously （P 〈 0.01 vs EAE model group）. It also decreased IFN -γ while increased IL- 13 secreted by lymphocyte （P 〈 0.01 vs EAE model group）. Conclusion Duhuo can alleviate CNS damage in EAE, inhibit the proliferation of lymphocyte and re- store the balance of Thl/Th2, thereby alleviating MS/EAE.

关 键 词：独活 实验性自身免疫性脑脊髓炎 多发性硬化 细胞因子 

分 类 号：R285.5[医药卫生—中药学]