白花败酱草体外抗肿瘤活性部位筛选  

The Anti-tumor Effect of the Extracts of Patrinia villosa Juss

作　　者：宋婷[1,2] 孙晖[2] 路娟[1] 李凤金 张颖智[1,2] 陈曦[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《时珍国医国药》2012年第10期2410-2412,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81173645);国家“重大新药创制”科技重大专项“十二五”课题(No.2012ZX09301-002-001)

摘　　要：目的建立白花败酱草活性部位的提取方法,初步筛选出白花败酱草抗肿瘤活性部位。方法采用四甲基偶氮唑蓝(MTT)比色法。结果白花败酱草粗提物能够显著诱导Hela和Mcf-7细胞发生凋亡;80%乙醇树脂部位对Hela、Mcf-7和Hct-8细胞具有显著的抑制作用,IC50值分别为11.90,12.55,15.61 mg·L-1,对EJ细胞的抑制作用次之,IC50值为65.32 mg·L-1,其他部位的抑制作用较弱或无抑制作用;紫外显色法测得80%乙醇树脂部位中总皂苷含量是61.81%。结论 80%乙醇树脂部位主要成分是皂苷,其具有明显的体外抗肿瘤作用。Objective To estimate the anti - tumor activity of the extracts of Patrinia villosa Juss. Methods MTT assay was ap- plied to screen the anti - tumor effect of the extracts of Patrinia villosa Juss on different tumor cell lines in vitro. Results Different extracts bad various inhibitory effects on different tumor cell lines. Hela, Mcf - 7, EJ and Het - 8 cell lines had sensibility to the crude extracts of Patrinia villosa Juss. 80% alcohol elution from mastic part had remarkable anti - tumor activity in vitro. The half inhibition concentrations were 9.29,10.21 and 10.33 mg · L^-1 to Hela,Mcf- 7 and EJ of human respectively, and other parts of elution mastic part had little inhibitory effect on these cell lines. The contents of total saponins and total flavonoids of 80% alcohol - soluble part were 61.81% and 8.69% respectively. Conclusion 80% alcohol - soluble part, which is considered as the active parts of Patrinia villosa Juss, has considerable anti - tumor effect.

关 键 词：白花败酱草 总皂苷 抗肿瘤 活性筛选 

分 类 号：R285.5[医药卫生—中药学]