抗肝纤冲剂对免疫损伤小鼠肝纤维化肝组织病理改变的影响  

Effect Kang Gan Xian Chong Ji on Pathological Changes of Hepatic Fibrosis Tissue of the Mice Induced by Immunological Injury

作　　者：肖和杰[1,2] 柳长柏[3,4] 张艳琼[4] 石次国[1,2] 陈钢[1,2] 杜杨红[1,2] 石慧[1,2] 吴江锋[3,4] 

机构地区：[1]葛洲坝中心医院 [2]三峡大学第三临床医学院,湖北宜昌443003 [3]三峡大学分子生物学研究所,湖北宜昌443002 [4]三峡大学医学院,湖北宜昌443002

出　　处：《时珍国医国药》2012年第10期2420-2422,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81170412);葛洲坝集团公司重大科研项目(No.2007KJ-25);湖北省卫生厅青年科技人才项目(No.QUX2010-28);湖北省宜昌市科研立项[宜科发(2007(24),No.A2007302-38)]

摘　　要：目的观察抗肝纤冲剂对猪血清所致的免疫损伤肝纤维化小鼠肝组织的病理学影响。方法将75只雌性Balb/c小鼠分为5组,每组15只:正常对照组,肝纤维化模型组,秋水仙碱治疗组,抗肝纤冲剂低剂量组,抗肝纤冲剂高剂量组。除正常组外,其余各组均采用腹腔注射猪血清的方法制备肝纤维化小鼠模型。造模的同时分别用药物对小鼠进行干预治疗7周。7周末处死小鼠,取血、肝脏及脾脏。用全自动生化仪测定血清肝功能指标;肝组织固定、切片、HE染色,于光镜下观察肝脏病理变化。结果药物治疗组的小鼠一般状况有所改善,死亡率降低。抗肝纤冲剂治疗组与模型组、秋水仙碱组相比,血清肝功能指标ALT、AST显著降低(P<0.01,P<0.05);肝组织的病理学显示,抗肝冲剂治疗组与模型组相比,肝细胞炎症减轻。结论抗肝纤冲剂可以显著减轻免疫损伤小鼠肝纤维化的肝组织的病理学损伤。Objective To observe the effect of KangGan Xian Chongji on pathological changes of the hepatic fibrosis mice immu- nological injury. Methods Female Balb/c mice 75 were divided into 5 groups randomly： control group, hepatic fibrosis model group, eolehicine group,low and high - dosage therapeutic groups of Kang Gan Xian Chong Ji. 15 mice in each group. The hepatic fibrosis model induced by intraperitoneal injection of pig serum （0.5ml/onee, once a week, total 7weeks）. Therapeutic group mice were treated by the colchicine or Kang Gan Xian Chong Ji, once a day, for 7 weeks. The mice were killed and the blood,liver and spleen from mice at the end of the seventh week. The serum biochemical indicaters of the mice were detected by the full automatic biochemical analyzer, and pathological changes of liver were observed with HE stain under microscope. Results The general con- dition drug was better, and the mortality rate decreased. The level of ALl＇, AST were obvious in Kang Gan Xian Chong Ji groups compared with model group （ P 〈 0.05 ）. The hepatic pathology indicated that the imflammatory reaction of hepatic cells in the groups of Kang Gan Xian Chong Ji decreased compared with the model group. Conclusion Kang Gan Xian Chong Ji can obviously decrease the hepatic fibrosis of mice induced by immunological injury.

关 键 词：肝纤维化 抗肝纤冲剂 

分 类 号：R285.5[医药卫生—中药学]