贯叶连翘提取物中金丝桃苷含量的RP-HPLC法测定  

Determination of Hyperin in Hypericum perforatum L. Extract by RP-HPLC

作　　者：韩霖[1,2] 刘妍如[2,3] 杨建云[2] 肖炳坤[2] 黄荣清[2] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《时珍国医国药》2012年第10期2432-2434,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81072613)

摘　　要：目的建立测定贯叶连翘提取物中金丝桃苷含量的高效液相色谱法。方法采用Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液(15∶85)为流动相,流速1.2 ml/min,检测波长256 nm;柱温为35℃。结果金丝桃苷检测浓度在11.4～91.2μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 9);平均回收率为100.7%,RSD为1.37%。结论该法快速、简便、准确,重复性好,可用于贯叶连翘提取物中金丝桃苷的含量测定。Objective To develop an RP - HPLC method for the determination ot hypenn m ttypertcum perJoratum L. extracts. Methods The RP -HPLC system consisting of Agilent ZORBAX SB -C18 （250 mmx 4.6 mm, 5 μm） column was adopted and the mobile phase was acetonitrile-0.1% phosophoric acid（ 15： 85） at the flow rate of 1.2 ml/min; the detection wavelength was 256 nm and the column temperature was 35℃. Results The linear range for hyperin was 11.4 - 91.2 p,g/ml （ r =0. 999 9）. The average recovery was 100.7% （RSD = t. 37% ）. Conclusion This method is simple,rapid,accurate,and it can be used for the quality control of the Hypericum pe（foratum L. extract.

关 键 词：贯叶连翘 金丝桃苷 反相高效液相色谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]