牛膝“引药下行”对双氯芬酸钠抗大鼠急性关节炎作用的影响  

The “Drug-guide” Effect of Achyranthes bidentata Bl. on Anti-inflammatory Action of Diclofenac Sodium

作　　者：刘国生[1] 孙备[1] 吕凌[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,安徽合肥230038

出　　处：《时珍国医国药》2012年第10期2443-2445,共3页Lishizhen Medicine and Materia Medica Research

基　　金：安徽省自然科学基金(No.070413120)

摘　　要：目的研究具有"引药下行"作用的中药牛膝对化学药物双氯芬酸钠抗炎作用的影响。方法大鼠随机分为正常对照组、模型对照组、牛膝组、双氯芬酸钠组、牛膝+双氯芬酸钠组。采用角叉菜胶法制备大鼠急性关节炎模型,容积法测定大鼠足跖体积,HPLC法测定大鼠血浆及组织中双氯芬酸钠含量,ELISA法测定大鼠血浆及组织中PGE2水平。结果牛膝和双氯芬酸钠合用后在0.5,1.5,3,5 h的抗炎作用均强于单用双氯芬酸钠组(P<0.05);牛膝可以提高双氯芬酸钠的组织分布浓度,使双氯芬酸钠的AUCtissue/AUCblood提高了60%;双氯芬酸钠与牛膝合用后,炎症组织中的PGE2浓度下降更为明显,但血浆中的PGE2浓度反而比单用双氯芬酸钠时有所升高。结论牛膝能够提高双氯芬酸钠在下肢炎症组织的靶向分布,牛膝对双氯芬酸钠具有靶向引导作用。Objective To research the ＂ drug- guide＂ effect of Achyranthes bidentata B1. -an traditional Chinese medicine （TCM） on the anti - inflammatory action of diclofenac sodium. Methods The rats were divided into five groups ： normal group, model group, Achyranthes bidentata B1. group, diclofenac sodium group, Achyranthes bidentata BI. ＋ diclofenac sodium group. The acute inflammatory model was induced by carragenin, the volume of foot was tested by volume displacement method, the di- clofenac sodium concentration in plasma and tissue was determined by HPLC, the PGE2 level in plasma and tissue was determined by ELISA. Results When used together, Achyranthes bidentata B1. could enhance the anti - inflammatory effect of diclofenac so- dium at 0.5, 1.5, 3, 5h after giving drugs; Achyranthes bidentata B1. could increase the diclofeuac sodium concentration in tar- geted tissue, and the AUCtissuo/AUCblood increased by 60% ; the PGE2 level was decreased more obviously in tissue than plasma. Conclusion Achyranthes bidentata B1. can enhance the diclofenac sodium distribution in inflammatory tissue, so it may have tar- geted guide effect to other drugs.

关 键 词：牛膝 双氯芬酸钠 急性关节炎 靶向引导 

分 类 号：R285.5[医药卫生—中药学]