理冲汤对体外培养人子宫肌瘤细胞增殖和形态的影响  

Effects of Lichong Decoction on the Proliferation and Morphology of Human Uterine Leiomyoma Cells in Vitro

作　　者：郑九波[1] 李冬华[1] 张南[2] 王亚松[1] 邹小丽[1] 韩虹娟[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]河南中医学院,河南郑州450003

出　　处：《中医药导报》2012年第10期12-15,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然基金项目(81073096);北京市自然科学基金资助项目(7082015);北京市属高等学校人才强教深化计划"中青年骨干人才培养计划"项目(PHR 201008403)

摘　　要：目的:研究理冲汤对体外培养人子宫肌瘤细胞增殖和形态改变的影响。方法:进行人子宫肌瘤细胞原代、传代培养及鉴定后,加入理冲汤含药血清,继续培养,CCK-8法检测理冲汤对体外培养人子宫肌瘤细胞增殖的影响,HE染色镜下观察子宫肌瘤细胞形态的改变。结果:理冲汤各组对体外人子宫肌瘤细胞的增殖有明显抑制作用(P<0.01),光镜下显示,与对照组相比,出现胞浆减少或萎缩,细胞死亡。结论:理冲汤治疗子宫肌瘤的作用机制可能与抑制子宫肌瘤细胞的增殖、改变肌瘤细胞的形态有关。Objective： To investigate the effects of Lichong Decoction on the proliferation and morphology of human uter- ine leiomyoma cells in vitro. Methods： The primary culture and subculture of the human uterine leiomyoma cells were per- formed and identified. The cells were treated with Lichong Decoction. The proliferation of cultured human uterine leiomyoma ceils was detected by CCK-8 assay. The morphological changes of cultured human uterine leiomyoma ceils were observed un- der microscope after HE stain, most of the cytoplasm appear to atrophy and a large number of cells show death phenomenon. Results： Lichong Decoction showed a obvious inhibitory action on proliferation of human uterine leiomyoma cells （P〈O.O1）and the great morphological changes under light microscope observation. Compared with untreated cultures,the groups of Lichong Decoction inhibited the proliferation, reduced the cytoplasm and induced apoptosis of the cultured leiomyoms cells.Conclusion： The anti-tumor effect of Lichong Decoction on uterine leiomyoma might be related to the inhibition of proliferation and the morphological changes.

关 键 词：子宫肌瘤 细胞培养 理冲汤 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]