低抗凝肝素来源的低分子肝素的研究  

STUDIES ON LOW MOLECULAR WEIGHT HEPARIN FROM LOW ANTICOAGULANT ACTIVITY HEPARIN

作　　者：杨立波[1] 张天民[1] 

机构地区：[1]山东医科大学生化药物研究室

出　　处：《中国医药工业杂志》1990年第4期178-178,共1页Chinese Journal of Pharmaceuticals

摘　　要：低分子肝素是由商品肝素分得的低分子组分,或利用化学解聚法等将其裂解制得。本实验以肝素生产的副产品——低抗凝肝素为原料,经亚硝酸控制解聚和凝胶层析制备低分子肝素。<Absrtact>Low molecular weight heparin (LMWH) can ba prepared by either fractionation or chemical depoly-merization of commercial heparin. We prepared LMWH from low anticoagulant activity heparin (LAAH), a side product accompanying commercialhe-parin production, by depolymerization with nitrous acidcombined with gel chromatography·; Its anticoag-ulant、 activity,physical and chemical parameters were determined. The ntithrombotic activity was eva-luated by the formation of thrombus in common ca-rotid artery in experimental rat thrombus model. The results showed that the anticoagulant ac-tivity of LMWH obtained was 30.33 a/mg, the eve-rage molecular weight was 5200d. The content ofglucosamine, hexuronic acid and sulfate was lowerthan that of LAAH. This implied that certain struc-ture changes had occurred, however, the UY and IRspectra of LMWH suggested that its main structure remained intact.The antithrombotic action of LMWH was stron-ger than LAAH and commercial heparin and theaction was dose dependent.LMWH obtained from LAAH not only costs less. but also might have more favorable clinical potential.

关 键 词：肝素 低分子肝素 生产 疗效 

分 类 号：TQ463[化学工程—制药化工] R973.2[医药卫生—药品]