4种中药多糖及胸腺肽对U14宫颈癌荷瘤鼠脾脏和瘤内免疫影响的比较  

Effects of thymopeptide and 4 polysaccharides isolated from Chinese medicine on immune functions of spleen and tumor tissues in U14 cervical cancer-bearing mice

作　　者：罗惠娟[1] 徐建平[1] 黎清[1] 谢杏美[1] 陈瑞平[1] 

机构地区：[1]暨南大学第一附属医院妇产科,广东广州510632

出　　处：《中国病理生理杂志》2012年第10期1895-1900,共6页Chinese Journal of Pathophysiology

基　　金：广东省中医药局资助科研项目(No.2010116)

摘　　要：目的:比较4种中药多糖及胸腺肽对U14宫颈癌荷瘤鼠肿瘤生长、脾增生、脾内T淋巴细胞(TLC)、自然杀伤细胞(NK)及瘤组织内Th1/Th2的影响,试图找到对宫颈癌有相对特异性的免疫增强剂。方法:建立U14宫颈癌荷瘤鼠模型,用生理盐水、中药多糖或胸腺肽连续21 d灌胃,处死后检测治疗组及对照组的抑瘤率和脾脏指数,采用流式细胞术和免疫组化法分析脾内TLC和NK细胞亚群以及瘤组织内IFN-γ和IL-10含量。结果:肿瘤抑制率排序:当归多糖(ASP)<人参多糖(PGP)<胸腺肽<枸杞多糖(LBP)<灵芝多糖(GLP),GLP、LBP和胸腺肽组与肿瘤对照组相比差异有统计学意义;PGP和ASP组与肿瘤对照组相比无显著差异。脾脏指数:ASP<肿瘤对照组<胸腺肽组<GLP<PGP<LBP;CD4/CD8增强排序:PGP<ASP<GLP<胸腺肽组<LBP;CD49b+NK增生排序:ASP<胸腺肽组<LBP<GLP<PGP,组间差异均有统计学意义。瘤组织内IFN-γ和IL-10检测显示:胸腺肽、LBP、GLP组Th1/Th2均向Th1漂移,程度依次减弱,PGP和ASP组无明显Th1漂移。结论:LBP、GLP和胸腺肽对U14宫颈荷瘤鼠的肿瘤生长有明显抑制作用,并能增强荷瘤鼠的免疫功能。AIM： To identify the enhancement of relatively specific immune responses by thymopeptide and 4 polysaccharides isolated from traditional Chinese medicine in the mice bearing cervical cancer. METHODS： The model of cervical cancer - bearing mice was established by implantation of cervical cancer U14 cells into the armpit of the fight forelimb of the mice. Beginning from the 2nd day of tumor implantation, 4 polysaccharides [ including Lycium barbarum poly-sacchaxide （ LBP）, Angelica sinensis polysaccharide （ ASP）, Ganodema lucidum polysaccharide （GLP） and Panax ginseng polysaccharide （PGP） ], thymopeptide or saline solution were intragastric administered for 21 days according to the experimental design. The animals were then killed, the tumor inhibitory rate and spleen index in treatment groups were compared with those in control group. The splenic T -lymphocytes （TLC） and natural killer （NK） cells, and the content of IFN -γ and IL- 10 in the tumor tissues were also determined by flow cytometry and immunohistochemical methods. RESULTS： The order of tumor inhibitory rates was ： ASP 〈 PGP 〈 thymopeptide 〈 LBP 〈 GLP. The effects of GLP, LBP and thymopeptide were statistically different from that of control group. No difference of tumor inhibition between PGP/ASP groups and control group was observed. The order of spleen index was： ASP 〈 tumor control group 〈 thymopeptide 〈 GLP 〈 PGP 〈 LBP, that of CD4/CD8 enhancement was： PGP 〈 ASP 〈 GLP 〈 thymopeptide 〈 LBP, and that of CD49b＋ NK hyperplasia was： ASP 〈 thymopeptide 〈 LBP 〈 GLP 〈 PGP, with statistically significant differences among the groups. The changes of IFN -γ and IL- 10 in the tumor tissues showed that Th1/Th2 in thymopeptide group, LBP group and GLP group shifted to Thl. No shift to Thl in PGP group and ASP group was observed. CONCLUSION： LBP, GLP and thymopeptide show significant inhibitory effects on tumor growth in U14 cervical tumor - bearing mice and can enhance the immune functi

关 键 词：宫颈肿瘤 中草药 胸腺肽 

分 类 号：R711.74[医药卫生—妇产科学]