HPLC法检测大鼠血浆中氯吡格雷羧酸代谢物SR26334  

Determination of clopidogrel′s carboxylic acid metabolite(SR26334) in rat plasma by HPLC

作　　者：戴歌心[1] 罗顺斌[2] 林迦勒[1] 陈连国[2] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000 [2]温州医学院药学院,浙江温州325000

出　　处：《中国卫生检验杂志》2012年第10期2314-2316,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立大鼠血浆中氯吡格雷羧酸代谢物SR26334检测的高效液相色谱方法,研究SR26334的药代动力学。方法:血浆经高氯酸沉淀,以ZORBAX SB-C18为色谱柱;流动相为乙腈-水-0.1%TFA(三氟乙酸),流速为1.0 ml/min;检测波长为230 nm(0 min～4.5 min)、280 nm(4.5 min～6 min)。结果:SR26334浓度在0.5 mg/L～50 mg/L范围内线性关系良好(r=0.9999);定量下限为0.5 mg/L;低、中、高三个浓度的相对回收率100.34%～101.78%;日内RSD(相对标准偏差)和日间RSD均小于10%。结论:本方法准确可靠、简便快速,适用于大鼠血浆SR26334浓度的测定。Objective： To develop a high performance liquid chromatography method for the determination of SR26334 in rat plasma and study its pharmacokinetics. Methods： The plasma samples were simply pretreated by protein precipitation with perchloric acetate. The analytical column was ZORBAX SB -C18. The mobile phase was acetonitrile - water - 0.1% trifluoroacetic acid and the flow rate was 1.0 ml/min. The UV detection wavelength was 230 nm （0 min-4.5 min） and 280 nm （4.5 min - 6 min）. Results： Excellent liner relationship was obtained from the range of 0.5 mg/L to 50 mg/L （ r = 0. 9999） , the lower limit of quantification of SR26334 was 0.5 mg/L. The relative recoveries were 100.34% - 101.78% , the intra - day RSD and inter - day RSD were both less than 10%. Conclusion： The method was accurate, simple, rapid and could be used to determine the SR26334 concen- tration in rat plasma.

关 键 词：高效液相色谱紫外法 氯吡格雷 SR26334 血药浓度 药代动力学 

分 类 号：O657.72[理学—分析化学]