胶体金试纸法研究及在硝基呋喃类代谢物应用  

Application of colloidal gold method in determination of nitrofuran metabolites

作　　者：曹毅敏[1] 潘心红[1] 彭荣飞[1] 黎淑端[1] 许萍[1] 

机构地区：[1]广东省广州市疾病预防控制中心,广州510440

出　　处：《中国卫生检验杂志》2012年第10期2342-2343,2363,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:通过对胶体金试纸条的研究,并用该方法对硝基呋喃类代谢物残留量中呋喃妥因代谢物(AHD)进行测定。方法:用胶体金试纸法测定牛奶中硝基呋喃类代谢物残留量中呋喃妥因,与ELISA法进行比较。结果:本方法的测定结果与ELISA法检测结果相符,总符合率达100%;另外,两种方法的测定结果经配对t检验,结果差异无显著性(P>0.05);回收率在85%～92%之间,相对标准偏差14.5%。结论:胶体金试纸条法的建立与研究,并在牛奶中硝基呋喃类代谢物呋喃妥因残留量的应用;具有快速、准确、省时、灵敏与简便的特点。Objective：To study the colloidal gold test strip and use this method to determine the level of nitrofu- ran in AHD residues. Methods： Colloidal gold test strip was used to determine the level of nitrofuran in AHD resi- dues in milk, and the result was compared with that of ELISA. Results： The determination result was consistent with that of ELISA, the coincidence rate was 100%. With paired t test, there was no significant difference between the results of the two methods（P 〉 0.05）. The recoveries were 85% -92%, the RSD was 14.5%. Conclusion： The colloidal gold test strip was rapid, accurate, time - saving, sensitive and handy in determination of nitrofuran residues in milk.

关 键 词：胶体金试纸法 硝基呋喃类 呋喃妥因代谢物 残留量 

分 类 号：O656.2[理学—分析化学]