InvA-PCR应用于磐安县钩端螺旋体病检测的研究  

Application of InvA-PCR in leptospirosis detection in Pan′an

作　　者：李钟梁[1] 孙继明[2] 姜理平[2] 应凯满[1] 郑柏福[1] 李夏[1] 陈岚岚[1] 张孟田[1] 

机构地区：[1]浙江省磐安县疾病预防控制中心,浙江磐安322300 [2]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2012年第10期2360-2363,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:针对InvA-PCR方法检测钩端螺旋体病的研究。方法:根据侵袭蛋白A基因(InvA)与卫生行业标准推荐的引物G1/G2对比,进行特异性与灵敏度研究,并应用于磐安鼠标本钩端螺旋体PCR检测。对磐安县2009分离菌株进行钩端螺旋体InvA-PCR和LS-PCR鉴定。结果:InvA-PCR具有较高的灵敏度和特异性,该引物可特异性扩增致病性钩端螺旋体DNA,对本实验所用的其他细菌不扩增。2009年分离的钩端螺旋体菌株进行PCR鉴定结果与血清学鉴定结果相符。对鼠标本进行InvA-PCR检测,InvA-PCR检测阳性率为5%。与卫生行业标准推荐的引物G1/G2对比,符合率为100%。结论:InvA-PCR检测方法可灵敏、特异地检测致病性钩端螺旋体,能正确有效地反应野生动物带毒率,为控制钩端螺旋体病提供依据。Objective：To study the application of InvA - PCR in leptospirosis detection. Methods： Study on specificity and sensitivity of InvA - PCR and LS - PCR were carried out based on comparison of InvA and G1/G2 （ the primers were recommended by health industry standard） , then InvA - PCR and LS - PCR were applied in lep- tospirosis identification of isolates in 2009 in Pan＇an. Results： InvA -PCR had high sensitivity and specificity, and InvA can lead to specific amplification of pathogenic leptospira DNA, while the amplification was not found in other pathogens in this study. The identification result of leptospira isolates in 2009 by InvA - PCR was found to conform to that with serological method. When InvA -PCR was used on mice specimens, the positive rate was 5 %, which was the same as the G1/G2. Conclusion： In leptospira detection, InvA - PCR can reveal the virus carrying rate of wild animals correctly and effectively and provide basis for control of leptospirosis.

关 键 词：侵袭蛋白A基因 钩端螺旋体 聚合酶链反应 

分 类 号：R514.4[医药卫生—内科学]