血清中葡萄糖-6-磷酸异构酶抗原的检测在类风湿性关节炎中的意义  

The significance of serum glucose-6-phosphated isomerase antigens in rheumatoid arthritis

作　　者：郑静[1] 袁兆林[2] 郑玉芬[1] 陈佳喜[2] 

机构地区：[1]台州恩泽医疗中心(集团)浙江省台州医院检验科,浙江临海317000 [2]温州医学院,研究生浙江温州325000

出　　处：《中国卫生检验杂志》2012年第10期2398-2400,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨葡萄糖-6-磷酸异构酶(GPI)检测、及与抗环瓜氨酸多肽(CCP)抗体、类风湿因子(RF)联合检测在RA诊断中的价值。方法:用ELISA双抗体夹心法检测RA组、OA组和正常对照组GPI、抗CCP抗体和RF水平。结果:RA组GPI(1.9±1.3)mg/L、OA组(0.22±0.08)mg/L(P<0.01)、正常对照组(0.14±0.07)mg/L(P<0.01);GPI在RA活动组和非活动组差异有统计学意义(P<0.01);同时其对RA的灵敏度、特异度均高于抗CCP抗体、RF。经pearson检验,GPI与RA病人DAS28评分正相关(r=0.456,P<0.01)。结论:血清GPI对诊断RA、尤其是活动期RA有相对较高灵敏度和特异度、可作为RA诊断和评估的实验室指标。Objective：To explore the clinical value of Glucose -6 - Phospated isomerase （GPI） in the Rheum atoid Arthritis（RA）. To assess the diagnostic value of GPI, anti -cyclic citrullinated peptide antibodies（ anti - CCP） and Rheumatoid Factor（RF） for RA. Methods：The levels of GPI, anti - CCP and RF were detected in the se- rum samples of RA patient group, Osteoarthritis （OA） patient group and normal control group by sandwich ELISA methods. Results ： The level of GPI was higher in RA patient group [ （ 1.9 ± 1.3 ） mg/L ] than that in OA patient group [ （0.22 ±0.08）mg/L（P 〈0.01） ] and normal control group[ （0.14 ±0.07）mg/L（P 〈0.01 ） ] ,and there was also significant difference in the concentration of GPI between active group and inactive group of RA patients （P 〈 0.01 ） ; moreover,the sensitivity and specificity of GPI were also higher than anti -CCP and RF in RA patients. After the pearson analysis, GPI concentrations and DAS28 score of RA patients was positively related （ r = 0. 456, P 〈 0.01 ）. Conclusion： In the RA patients, especially in the active stage of RA patients, the level of serum GPI has a relatively high sensitivity and specificity. GPI can be used as a laboratory indicator to diagnose and assess the RA patients.

关 键 词：葡萄糖-6-磷酸异构酶 类风湿性关节炎 ELISA 

分 类 号：R593.22[医药卫生—内科学]