丹莪妇康煎膏对大鼠肝毒性的实验研究  

Studies on hepatotoxicity of rats induced by Dan' e Fukang Decocted Extract

作　　者：贺克[1] 刘姣[2] 李彩霞[1] 刘丽华[1] 姜秀娟[2] 

机构地区：[1]石家庄市第四医院药剂科,河北石家庄050011 [2]河北医科大学中医学院药剂科,河北石家庄050091

出　　处：《药品评价》2012年第26期21-23,共3页Drug Evaluation

基　　金：河北省科学技术项目<丹莪妇康煎膏与孕三烯酮胶囊对子宫内膜异位症的治疗作用及安全性比较研究>(10276105D-46)

摘　　要：目的:观察丹莪妇康煎膏对大鼠的肝脏毒性作用。方法:大鼠连续灌胃丹莪妇康煎膏(5.6g/kg、2.8g/kg)30d,同时设对照组。每5d称体重一次,末次给药后禁食12h,麻醉后取血,测定血清AST、ALT和ALP值,取肝脏称重并计算肝脏指数,HE染色观察病理改变。结果:丹莪妇康煎膏各剂量组大鼠体重增长均显著低于对照组(P<0.01);丹莪妇康煎膏高剂量组大鼠肝脏湿重与对照组相比无显著性差异(P>0.05),但肝脏指数却显著增加(P<0.05),低剂量组肝脏湿重及肝脏指数与对照组相比均无显著性差异(P>0.05);丹莪妇康煎膏各剂量组大鼠血清AST、ALT和ALP值与对照组相比均无显著性差异(P>0.05),大鼠肝脏组织无明显病变。结论:丹莪妇康煎膏连续灌胃30d,对大鼠肝脏无明显毒性。Objective： To study the hepatotoxicity of rats induced by Dan＇e Fukang Decocted Extract （DFD）. Methods： Wistar rats were treated with DFD（5.6g/kg, 2.8g/kg）for 30 days by gavage. After last administration, rats were sacrificed. Blood was taken from abdominal aorta, and the levels ofAST, ALT and ALP in serum were determined. Liver was weighted, liver coefficient was calculated, and the pathological changes of liver were determined by HE stain. Results： Compared with control group, body weight gaining in high-dose and low-dose DFD groups were significantly lower （P〈0.01）. Liver weight in high-dose DFD group was similar to control group （P〉0.05）, but liver coefficient in high-dose DFD group was higher than control group （P〈0.05）. Liver weight and coefficient in low-dose DFD group were similar to control group （P〉0.05）, and there was no significantly abnormal in liver tissue. Serum AST, ALT and ALP in high-dose and low-dose DFD groups were similar to control group （P〉0.05）. Conclusion： There was no significant hepatotoxicity induced by DFD.

关 键 词：丹莪妇康煎膏 肝毒性 天门冬氨酸氨基转移酶 丙氨酸氨基转移酶 碱性磷酸酶 

分 类 号：R285.5[医药卫生—中药学]