不同林分类型油松毛虫COⅠ基因变异分析  

Sequence Variation of COⅠ Gene of Dendrolimus tabulaeformis in Different Types of Stand

作　　者：夏明瑞[1] 周国娜[1] 高宝嘉[1,2] 

机构地区：[1]河北农业大学,保定071000 [2]河北北方学院,张家口075000

出　　处：《林业科学》2012年第9期171-175,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30771739)

摘　　要：昆虫和其他生物一样,在长期的生长发育和进化过程中保留了一些遗传特征,使物种得以延续,但同时也存在一些变异,以适应新的环境。这些遗传变异不仅仅存在于昆虫的种间,也存在于同种昆虫的不同种群之间（蔡青年等,2004;施立明,1990）。不同的寄主植物、地理隔离及其他环境条件都会对昆虫种群的遗传变异产生一定的影响。顾耘等（2002）利用RAPD技术对辣椒（Chili pepper）、烟草（Nicotianatabacum）和番茄（Solanum lycopersicum）上所寄生的棉铃虫（Helicoverpa armigera）进行分析,发现棉铃虫不同寄主种群已经存在明显的遗传分化;In order to clarify the effect of different stand types on the genetic structure of Dendrolinus tabulaeformis, we determined a 554 bp segment of the mitochondrial cytochrome oxidase subunit （COⅠ） gene sequence of D. tabulaeformis populations from of 4 types of stands, and then analyzed the sequence variability and genetic differentiation. The results showed that in the gene sequence of 544 bp, the average contents of A, T, C and G were 30.6%, 39.4%, 15.3% and 14.7%, respectively, and the contents of A＋T accounted for 70% of the total bases and were obviously higher than that of C＋G, with an obvious A/T bias. In this sequence fragment, 64 nucleotide sites showed variation and the variability was 11.7%. Genetic distance of the nucleotide sequences was 0.002-0.046, indicating a low genetic variation. A dendrogram was constructed by using NJ and MP method, and the results showed that the genetic differentiations were correlated with the type of stands. Furthermore, the Fst coefficients of gene differentiation were between -0.128 to 0.117, and the number of migrant per generation was all greater than 1, manifesting that there were to some degrees genetic differentiation and gene exchange between D. tabulaeformis populations from different types of stands.

关 键 词：油松毛虫 COⅠ基因 遗传分化 林分类型 

分 类 号：S763.421[农业科学—森林保护学] Q969[农业科学—林学]