油茶籽壳原花青素的分离纯化  

Study on Isolation and Purification of Procyanidins from the Tea(Camellia oleifer) Seed Shell

作　　者：余红军[1,2] 郑生宏 李立祥[2] 

机构地区：[1]浙江绿世界生物工程有限公司,浙江湖州313300 [2]安徽农业大学茶叶生物化学与生物技术教育部,农业部重点实验室,安徽合肥230036 [3]浙江丽水市农业科学研究院,浙江丽水323000

出　　处：《食品与发酵工业》2012年第9期196-199,共4页Food and Fermentation Industries

基　　金：安徽省高等学校“十五”优秀人才计划项目资助([安徽省教育厅教秘人2005 79号])

摘　　要：利用Sephadex LH-20凝胶分离纯化油茶籽壳中的原花青素,并对洗脱后得到的样品进行分析鉴定。结果表明:采用60%的丙酮溶液洗脱得到的原花青素含量较高,可达57.3%;HPLC分析显示,从Sephadex LH-20洗脱下来的组分中含有原花青素的单体物质。The procyanidins from the tea（ Camellia oleifer） Seed Shell was isolated and purified with Sephadex LH-20, and the samples were analyzed and identified. The result showed that procyanidins which from the tea（ Ca- mellia oleifer） Seed Shell, was purified by Sephadex LH-20 using 60% acetone elution received higher levels of pro- cyanidins, the yield was 57.3%. The purified product was further analyzed by HPLC. It indicated that the Procyani- din monomer was existed in the samples.

关 键 词：SEPHADEX LH-20 油茶籽壳 HPLC 原花青素 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]