枯草芽胞杆菌XF-1的根围定殖能力分析  

Analyses for the colonization ability of Bacillus subtilis XF-1 in the rhizosphere

作　　者：刘庆丰[1] 熊国如[1,3] 毛自朝[2] 吴毅歆[2] 何月秋[1,2] 

机构地区：[1]云南农业大学,教育部农业生物多样性与病虫害控制重点实验室,昆明650201 [2]云南农业大学农学与生物技术学院,昆明650201 [3]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《植物保护学报》2012年第5期425-430,共6页Journal of Plant Protection

基　　金：云南省重点基金项目(2008CC024);农业部公益性行业(农业)科研专项(201003029);科技部国际科技合作项目(2009DF-A32360);云南省支撑计划项目(2009EB060)

摘　　要：采用抗生素标记菌株与盆栽试验研究了对大白菜根肿病具有良好防治效果的枯草芽胞杆菌XF-1的定殖动态及影响其定殖的因素,以便了解该菌株的生防机制及其商品化应用前景。结果表明,XF-1的定殖能力较强,能在大白菜根围、根表和根内长时间定殖,接种60天后,根围和根表定殖密度仍能稳定在103CFU/g土壤,根内定殖密度也仍稳定在102CFU/g根(鲜重)水平,根围和根表定殖趋势为先下降后上升再下降,最后趋于稳定;根内定殖动态则呈先上升后下降的趋势。XF-1在根部组织内的皮层薄壁细胞和导管内均有分布。定殖受土壤质量影响,灭菌土中定殖能力最强;施入菌液浓度越高,定殖菌体数越多;在16℃光照培养箱中28天后,定殖仍可达到105CFU/g土壤;施入马铃薯汁蔗糖液体培养基(PS)发酵的菌液后,XF-1在根围中的定殖量大于施入马铃薯汁葡萄糖液体培养基(PD)发酵的菌液。XF-1还能很好地在玉米根围定殖。Bacillus subtilis XF-1 had good control effects on clubroot of Chinese cabbage both in the greenhouse and in the field plots. Colonization dynamics and the impact factors of XF-1 by biotics marker and pot-culture were studied to understand the bacterial control mechanism and provide the theoretical ba- sis of commercialization. The greenhouse experiment results indicated that it could colonize in the rhizo- sphere, root surface and root of Chinese cabbage successfully. The survival number of B. subtilis XF-1 in the rhizosphere and root surface remained 103 CFU/g soil and 102 CFU/g inside the root after inoculation of 60 days. The trend of colonization in rhizosphere and root surface increased first, then decreased and increased again and finally stabilized. The trend of colonization inside the root was rising and then fall- ing. The 15-day-old root of Chinese cabbage was used to make paraffin section and observed the bacteri- um under microscope. The results showed that XF-1 cells were observed in parenchyma and trachea of the root, its ability of colonization was affected by temperature, concentration, media and soils. Its colo- ny number in sterilized soil was more than that in natural soil. The higher its suspension concentration,the more colony number was. XF-1 could colonize in Chinese cabbage rhizosphere with 105 CFU/g soil after 28 days in 16 ~C incubator. The colony number of Chinese cabbage rhizosphere was higher when po- tato juice sucrose （PS） was used than that when potato juice dextrose （PD） as a medium. XF-1 could also colonize in the rhizosphere of maize.

关 键 词：XF-1菌株 大白菜根肿病 定殖能力 影响因素 

分 类 号：S476[农业科学—农业昆虫与害虫防治]