用中空纤维液相微萃取-HPLC法测定人血浆中酒石酸美托洛尔的浓度  

Determination of the concentration of metoprolol tartrate in human plasma by HPLC coupled with hollow fiber liquid phase microextraction method

作　　者：宫雪菲[1] 马海英[1] 易丽昕[1] 刘亚非[1] 

机构地区：[1]中国医科大学附属第四医院药学部,沈阳110032

出　　处：《药学服务与研究》2012年第5期374-377,共4页Pharmaceutical Care and Research

基　　金：辽宁省科学技术计划项目(No.2011225020)

摘　　要：目的:建立中空纤维液相微萃取-HPLC法测定人血浆中酒石酸美托洛尔的浓度。方法:优化酒石酸美托洛尔液相微萃取法供给相和接受相的浓度、萃取时间、萃取温度、萃取转速和离子强度,血浆样品经中空纤维液相微萃取法萃取后,用HPLC法测定酒石酸美托洛尔的浓度。色谱柱:Agilent Zorbax Eclipse XDB-C18柱,流动相:甲醇-0.1%磷酸(40∶60),流速:1ml/min,激发波长:227nm,发射波长:305nm,柱温:30℃。结果:酒石酸美托洛尔在2～125ng/ml线性关系良好,低、中、高三种浓度(5、20、100ng/ml)的日内、日间精密度均<10%,回收率分别为(87.1±7.3)%、(92.6±5.8)%和(89.1±2.5)%。结论:中空纤维液相微萃取-HPLC法适用于测定血浆样品中酒石酸美托洛尔的浓度。Objective： To establish a HPI.C coupled with hollow fiber liquid phase microextraction（HF LPME） method for determination of the concentration of metoprolol tartrate in human plasma. Methods： The concentrations of donor phase and receptive phase,time,temperature and rotary speed of extraction, and concentration of NaC1 in HF-LPME were optimized. The hollow fiber was placed in plasma sample solution to perform microextraction, and then the extract was analyzed by HPLC method with fluorescence detection on Agilent Zorbax XDtS-C~ column. The mobile phase consisted of methanol-0. 1 ~ phos phoric acid（40 ： 60） with a flow rate of 1 ml/min. The excitation wavelength was 227 nm and the emission wavelength was 305 nm. The column temperature was 30 ~C. Results： Metoprolol tartrate was in good linearity within the range of 2-125 ng/ml. The intra-day and inter-day RSD of low, middle and high concentrations （5,20,100 ng/ml） were all less than 10%, and recoveries were （87.1 ± 7.3） %, （ 92.6± 5.8） % and （ 89.1 ± 2.5 ）% , respectively. Conclusion： HPLC coupled with HF-I.PME method is suitable for analysis of concentration of metoprolol tartrate in human plasma.

关 键 词：中空纤维液相微萃取 酒石酸美托洛尔 色谱法 高效液相 含量测定 

分 类 号：R972.4[医药卫生—药品] R927.2[医药卫生—药学]