抗NDVF蛋白单克隆抗体在鹅源新城疫病毒感染快速检测中的应用  

Application of Monoclonal Antibody Against Fusion Protein of Newcastle Disease Virus in Rapid Detection of Goose Newcastle Disease Virus Infection

作　　者：金文杰[1] 王倩倩[1,2] 崔平福[1,3] 邵红霞[1] 秦爱建[1] 

机构地区：[1]扬州大学兽医学院,教育部禽类预防医学重点实验室,江苏扬州225009 [2]扬州市畜牧兽医站,江苏扬州225009 [3]泰州出入境检验检疫局,江苏泰州225300

出　　处：《中国家禽》2012年第20期29-32,共4页China Poultry

基　　金：江苏省“青蓝工程”项目;教育部创新团队项目(IRT0978);江苏省优势学科项目

摘　　要：自1997年以来,鹅源新城疫病毒(GNDV)的感染已经成为养鹅业最重要的疾病之一。本研究以抗新城疫病毒融合蛋白单克隆抗体为介导,建立了快速检测鹅源新城疫病毒感染的间接免疫荧光技术(IFA)。所建立的方法特异性强,与传染性法氏囊病病毒、马立克氏病病毒及小鹅瘟病毒等均不出现交叉反应;用100TCID50GNDV感染鸡胚成纤维细胞,结果显示在感染后18h就可以检测到GNDV。与技术要求高、操作繁琐的RT-PCR以及病毒分离鉴定相比,IFA检测鹅源新城疫病毒感染具有快速、简单、特异等优点,具有很好的应用前景。Since 1997,goose Newcastle disease virus （GNDV）infection has been one of serious diseases in goose flocks. The monoclonal antibody against NDV fusion protein was used to establish an indirect ＇immunofluorescence assay （IFA） to rapidly detect the infection of GNDV. This method was specific and showed no cross reaction with infectious bursal disease virus, Marek＇s disease virus and gosling plague virus. 100 TCID50 GNDV were used to infect chicken embryo fibroblast cells which were detected at 18 h post-infection by IFA. Compared with the cumbersome RT-PCR and virus identification, IFA showed the characteristic of rapid, simple and good specification in detection of GNDV infection and had a good application prospect.

关 键 词：抗NDV F蛋白单克隆抗体 鹅源新城疫病毒(GNDV) IFA 

分 类 号：S855.3[农业科学—临床兽医学]