牛传染性鼻气管炎病毒VP22基因的克隆及序列分析  

Cloning and sequence analysis of the VP22 gene of Infectious bovine rhinotracheitis virus

作　　者：霍志云[1] 胡嘉欣[1] 童钦[2] 高维凡[1] 王炜[2] 武华[2] 

机构地区：[1]沈阳农业大学,沈阳110161 [2]中国农业科学院特产研究所,吉林吉林132109

出　　处：《黑龙江畜牧兽医》2012年第11期88-91,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：2010农业公益性行业科研专项(201003060)

摘　　要：为了研究牛传染性鼻气管炎病毒(IBRV)即Ⅰ型牛疱疹病毒(BoHV-1)VP22基因及其功能,试验采用设计的特异性引物通过PCR法对VP22基因进行扩增,并成功构建了VP22克隆载体,经序列测定后,用DNAStar软件对该序列和已发表的IBRV参考株、5型牛疱疹病毒(BoHV-5)、Ⅰ型人单纯疱疹病毒(HSV-1)、马立克病毒Ⅰ型(MDV-1)和伪狂犬病毒(PRV)的VP22基因序列及氨基酸序列进行比对分析。结果表明:分离自我国的BoHV-1与NCBI上发表的参考株的基因同源性为99.6%,氨基酸同源性为100%;4种疱疹病毒VP22的N端氨基酸相似性不高;BoHV-1和BoHV-5VP22基因和氨基酸序列同源性分别为81.9%和76.4%;BoHV-1 VP22基因的密码子偏嗜性较均一。To study the VP22 gene of infectious bovine rhinotracheitis virus （IBRV）, namely, BoHV - 1, and to investigate its function. VP22 gene was amplified by PCR method with designed specific primers, and then VP22 cloning vector was successfully constructed. After sequence determination, VP22 gene sequence and amino acid sequence were compared and analyzed by using DNAStar software among published IBRV reference strain, bovine herpesvirus type 5 （ BoHV -5 ） , herpes simplex virus type 1 （ HSV - 1 ） , Marek＇ s disease virus type 1 （ MDV - 1 ） , and pseudorabies virus （PRV）. The results showed that the gene and amino acid sequence homologies between isolated BoHV - 1 and reference strains were 99.6% and 100%, respectively. The similarities of N - terminal amino acid sequences of the VP22 gene were not higtl among four kinds of herpesvirus. VP22 gene and amino acid sequence homologies between BoHV - 1 and BoHV -5 were 81.9% and 76.4%, respectively. Meanwhile, the eodon bias of BoHV - 1 VP22 gene is uniform.

关 键 词：牛传染性鼻气管炎病毒 VP22基因 克隆 序列比对 

分 类 号：Q785[生物学—分子生物学]