树上干杏染色体制片优化及核型分析  

Optimization of Chromosome Sectioning and Karyotype Analysis of Apricot Cultivar “Shushanggan”

作　　者：刘敏[1] 王卉[1,2] 宁慧霞[1] 康喜亮[1] 努尔波拉提[1] 周黎[1,2] 王晓军[1] 

机构地区：[1]中国科学院新疆理化技术研究所,乌鲁木齐830011 [2]中国科学院研究生院,北京100039

出　　处：《西北农业学报》2012年第10期79-83,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(81102737);中科院"西部行动计划高新技术项目"(KGCXZ-YW-509)

摘　　要：比较不同取材时间,预处理和解离方法对树上干杏染色体制片的影响,观察1 000个根尖细胞,比较不同部位中期细胞和适宜核型分析的中期细胞所占比例。结果显示,取材时间为8:30,用0.002mol/L 8-羟基喹啉预处理4h制片所得染色体分散效果最佳。核型公式为2n=2 N=16m,核型不对称系数(As.K)为53.66%,属4A类型,核型对称性程度高,表明树上干杏在进化中处于偏向原始类型。The different sampling time,the methods of the pretreatment and dissociation in chromosome sectioning of apricot cultivar ＂shushanggan＂ were compared.1 000 top root cells were observed,the proportions of metaphase cells and the metaphase cells which were suitable for karyotype analysis were compared.The results showed that the best experimental protocol for karyotype analysis of apricot cultivar ＂shushanggan＂ was the better sampling time at 8：30,the better pretreatment with 0.002 mol/L 8-Hydroxyquinolin for 4 h.The karyotype analysis revealed that the karyotype of apricot cultivar ＂shushanggan＂ was 2n=2N=16m and its index of As.k was 53.66%,the karyotype asymmetry belonged to 4A type,its karyotype was symmetrica1,which suggested that it was relatively primitive forms.

关 键 词：树上干杏 核型分析 染色体数目 

分 类 号：S662.2[农业科学—果树学]