灰仓鼠多房棘球蚴腹腔动物模型的建立  

Establishment of an animal model of peritoneal Echinococcus muhilocularis infection in Cricetulus migratorius

作　　者：刘斌 徐艺玫 燕顺生 史深 麦丽开 廖力夫 

机构地区：[1]新疆实验动物研究中心,乌鲁木齐830002

出　　处：《中国实验动物学报》2012年第5期25-28,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家"十一五"科技支撑计划重点项目(No.2009BAI83B02)

摘　　要：目的观察多房棘球蚴(Echinococcus muhilocularis E.m)在灰仓鼠腹腔内的生长发育规律,制作E.m灰仓鼠动物模型。方法 2000个E.m原头节/只剂量腹腔接种灰仓鼠,接种后10、15、18、22、39、60、80和100 d,测量包囊重量和抗体,计算包囊重与灰仓鼠体重的包囊系数,观察多房棘球蚴在灰仓鼠体内的发育规律。分别按100个/只、500个/只和2000个/只剂量腹腔接种灰仓鼠各10只,100 d后剖检比较不同感染剂量的包囊发育情况和感染率。结果 62只灰仓鼠中有59只感染了多房棘球蚴。18 d可观察到成熟原头节,感染率达到100%,15 d出现抗体阳性,60 d时包囊系数达到15%以上;100、500和2000三种剂量的感染率100%;结论建立的灰仓鼠腹腔多房棘球蚴动物模型具有感染率高,感染周期短,包囊生长发育和抗体滴度变化易观察,感染60 d可作为E.m动物模型成模观察期。包囊系数,抗体滴度和包囊发育变化可作为造模的主要参考指标。雌性灰仓鼠造模优于雄性。Objective To establish an animal model of Echinococcus muhilocularis（E.m） infection in Cricetulus migratorius,and observe the growth of E.m in the peritoneal cavity of C.migratorius.Methods C.migratorius was inoculated into the peritoneal cavity in a dose of 2000 cephalomeres of E.m in each animal.The cyst weight and positive antibody were measured,and the cyst weight/body weight ratio was calculated.The growth of E.m was observed in the C.migratorius at 10,15,18,22,40,60,80,100 days after inoculation.Growth and the infection rate of E.m cysts was compared at 100 days after inoculation into 10 animals at a dose of 100,500 or 2000 E.m cephalomeres,respectively.Results Fifty nine of the 62 C.migratorus were infected by E.m.Mature E.m cephalomeres were observed at 18 days after inoculation and the infection rate reached 100%.Positive antibody was observed at 15 days after inoculation.The cyst weight/body weight ratio was over 15% at 60 days after inoculation.A 100% infection rate was observed at 100 days after inoculation at the three doses.Conclusions An animal model of peritoneal Echinococcus muhilocularis infection in Cricetulus migratorius is successfully established,with the characteristics of high infection rate,short period of infection,easy observation of the growth of E.m and antibody titer on these animal models.Sixty days after inoculation of E.m can be used as observation period of the animal modeling in C.migratorius.The cyst weight/body weight ratio,antibody titer and level of cyst growth can be used as the major reference indicators of E.m infection modeling.Females are better than males for making animal models of E.m infection in C.migratorius.

关 键 词：多房棘球蚴 灰仓鼠 包囊系数 动物模型 抗体 感染 

分 类 号：Q95-33[生物学—动物学] R816.1[医药卫生—放射医学]