Anti-complement constituents of Commelina communis and their targets in complement activation cascade  

鸭跖草的抗补体活性成分及其作用靶点(英文)

作　　者：金家宏[1] 程志红[1] 陈道峰[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海201203

出　　处：《Journal of Chinese Pharmaceutical Sciences》2012年第6期577-581,共5页中国药学（英文版）

基　　金：National Natural Science Foundation for ExcellentYouth (Grant No.30925042);State Key Program for the InnovativeDrugs from the Ministry of Science and Technology (Grant No.2009ZX09502-013 and 2009ZX09301-011)

摘　　要：Anti-complement activity guided fractionation led to the isolation of 24 compounds from Commelina communis.Bioassay showed that six compounds inhibited the classical pathway and alternative pathway with CH 50 values of 0.12-1.44 mM and AP 50 values of 0.28-7.05 mM,respectively.Preliminary mechanism studies demonstrated that quinovic acid acted on C1q,C2,C3,C4,C5 and C9 components of the complement system,β-sitosterol interacted with C3 and C4,（＋）-catechin-3-O-β-Dgluco（2-cinnamoyl）-pyranoside,p-cresol and 6-methoxy-3-methylbenzene-1,2,4-triol blocked C1q,C2,C3,C5 and C9.采用抗补体活性导向分离法从中药鸭跖草中分离鉴定了24个化合物, 活性测试结果显示6个化合物对补体系统的经典途径和旁路途径显示出不同程度的抑制作用 (CH500.12-1.44 mM, AP500.28-7.05 mM)。作用靶点研究显示喹诺酸作用于补体系统的C1q, C2, C3, C4, C5 和 C9组分; β-谷甾醇作用于C3 和 C4组分; (+)-儿茶素-3-O-β-D-(2-肉桂酰基)-葡萄糖苷、对甲基苯酚和6-甲氧基-3-甲基-1,2,4-苯三酚均作用于C1q, C2, C3, C5 和C9组分。

关 键 词：Commelina communis Chemical constituents Complement inhibitor Action targets 

分 类 号：R96[医药卫生—药理学]