高碘诱导小鼠海马组织NOS阳性神经元凋亡  

EXCESSIVE IODINE INDUCES NITRIC OXIDE SYNTHASE POSITIVE NEURONS APOPTOSIS IN HIPPOCAMPUS OF MICE

作　　者：王崇[1] 张松筠[2] 杨长春 

机构地区：[1]河北医科大学第二医院检验科,河北石家庄050000 [2]河北医科大学第二医院内分泌科,河北石家庄050000 [3]武装警察部队总医院内一科,北京100039

出　　处：《河北医科大学学报》2012年第10期1117-1119,F0002,共4页Journal of Hebei Medical University

基　　金：河北省科技厅科研指导课题(062761830)

摘　　要：目的观察高碘喂养小鼠海马组织一氧化氮合酶(nitric oxide synthase,NOS)阳性神经元的变化,探讨高碘对小鼠中枢神经系统损害及行为学的影响。方法选择昆明种初断乳健康小鼠50只(雌雄各25只),随机分为适碘及高碘2组,适碘组小鼠喂碘浓度为50μg/L蒸馏水,高碘组小鼠喂碘浓度为5 000μg/L的蒸馏水各180d,做Morris水迷宫行为学测试,再用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶[nicotinamide adenine dinucleotide phosphate(NADPH)-diaphorase,NADPH-d]组织化学染色法观察小鼠海马组织NOS阳性神经元的改变。结果高碘组小鼠海马组织NOS阳性神经元密度较适碘组明显降低,染色变浅;在行为学测试中,小鼠逃避潜伏期明显延长。结论高碘组小鼠海马组织NOS阳性神经元减少及染色变浅,为高碘喂养可对小鼠中枢神经系统造成损害提供了病理依据。Objective To observe the effect of excessive iodine on nitric oxide synthase （NOS） positive neurons in hippocampus of mice and explore the injury effect of excessive iodine on central nervous system. Methods Fifty mice, divided into two groups at random （ control group with appropriate iodine and trial group with excessive iodine） ,were fed with distilled water containing iodine 50p.g/L and 5 O00p.g/L respectively. The mice were killed at 6 months after Morris water maze. Nicotinamide adenine dinucleotide phosphate（NADPH） -diaphorase histochemistry was used to visualize NOS positive cells in hippocampus. Results The mean escape latency increased and NOS positive cells decreased significantly in excessive iodine group than in control group. Conclusion The reduce of NOS positive cells in hippocampus may be related to the injury of cognition in excessive iodine mice.

关 键 词：碘 海马 一氧化碳合酶 小鼠 

分 类 号：R329.481[医药卫生—人体解剖和组织胚胎学]