3种甲型流感快速检测试剂的性能评价  

Evaluation of three kinds of quick assay kits for detecting influenza A virus

作　　者：杨坤宇 陈慧 陈毅歆[2] 张毅 李庶甘 郑清炳[2] 杨剑 郑燕平 

机构地区：[1]厦门国际旅行卫生保健中心,福建厦门361012 [2]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心

出　　处：《中国国境卫生检疫杂志》2012年第5期317-319,327,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家质检总局科研基金项目(2012IK243)

摘　　要：目的比较3种甲型流感快速检测试剂的灵敏度。方法利用病毒分离培养液和入境人群的鼻咽拭子标本考查试剂盒的检测灵敏度。结果甲型流感抗原快速检测试剂(FluA-DOT)对8份病毒分离培养液的检测灵敏度达到10^(-2)HA,明显高于其他两种检测试剂;FluA-DOT对入境人群鼻咽拭子标本的检出率为91.67%,也远远高于其他两种检测试剂。结论甲型流感抗原快速检测试剂(FluA-DOT)的检测时间短、灵敏度高,适用于口岸现场的甲型流感快速筛查。Objective To evaluate the sensitivity of three different kinds of assay kits for detecting influenza A virus. Methods To analyze the sensitivity of assay kits for influenza A virus by using the viral culture liquid and nasopharyngeal swabs from entry-exit travelers as samples.Results All the kits were tested against 8 different kinds of strains,the FluA-DOT kit displayed a sensitivity of 10^（-2）HA,it was much better than other kits.The sensitivity of FluA-DOT reached 91.67%when the kit was used for the detection of Nasopharyngeal swab from the entry people,and it was also better than other kits.Conclusion The FluA-DOT kit need a short detection time and have a good sensitivity,it is fit for the rapid detection of influenza A virus at frontier ports.

关 键 词：甲型流感 快速检测 性能评价 

分 类 号：R511.7[医药卫生—内科学]