红三叶叶绿素锌钠的制备及其稳定性研究  

Preparation and stability of red clover sodium zinc chlorophyllin

作　　者：曾家豫[1] 武伟国[1] 孔维宝[1] 刘雄雄[1] 赵静[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2012年第6期82-88,共7页Journal of Northwest Normal University(Natural Science)

基　　金：西北师范大学与定西市校企合作项目

摘　　要：用乙醇浸提红三叶鲜青草预加工废水中的叶绿素,经皂化、浓缩、萃取、酸化、锌代和成盐等步骤制备叶绿素锌钠.利用Plackett-Burman实验对影响叶绿素锌钠制备的8个因素进行了评价,结果显示皂化pH值、锌代时间和硫酸锌用量为影响叶绿素锌钠制备的主要因素.以最陡爬坡实验确定Box-Benknken实验的中心试验点,在此基础上设计Box-Benknken实验,并对其结果进行响应面分析,得到叶绿素锌钠制备的最佳条件为皂化pH值11.50、锌代时间85.44min和硫酸锌用量25.40mL,对应的产率为31.76mg.L-1.叶绿素锌钠稳定性研究显示,本实验条件下制备的叶绿素锌钠的热稳定性、耐光性和耐氧化与还原能力良好,在pH值为6.0～10.0的环境中稳定性良好,在4%Fe2+或Al 3+的溶液中较为稳定,与蔗糖、葡萄糖或食盐等接触时稳定性好.Chlorophyll,extracted by ethanol from red clover fresh grass processing water,is made into sodium zinc chlorophyllin by saponification,concentration,extraction,acidification,zinc replacement and salification.Effects of eight variables are evaluated by Plackett-Burman design,the results indicate that salification pH value,zinc replacement time and zinc sulfate dosage are the primary factor to influence sodium zinc chlorophyllin preparation.Center test point of Box-Benknken design is determined by deepest ascent design,on that basis,Box-Benknken design is employed and its results are analyzed by response surface method,the optimal salification pH value,zinc replacement time and zinc sulfate dosage is 11.50,85.44 min and 25.40 mL,respectively,corresponding to yield of 31.76 mg·L-1.Stability study shows that sodium zinc chlorophyllin prepared under this experimental conditions is well resistant to heat,light,oxidant and reductant,has well stability in 6.0~10.0 pH value environment and for 4% Fe2＋ or Al3＋ solution,when contacted with sucrose,glucose or salt,also performs good stability.

关 键 词：红三叶 叶绿素锌钠 响应面分析 稳定性 

分 类 号：X703.1[环境科学与工程—环境工程]