荧光光谱法测定阿仑膦酸钠含量  

Fluorophotometric Determination of Sodium Alendronate

作　　者：吴悠[1] 杨甲甲[1] 蔡元丽[1] 段炯[1] 李晖[1] 

机构地区：[1]四川大学化学与工程学院,成都610065

出　　处：《理化检验（化学分册）》2012年第10期1184-1186,1189,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：基于在pH 8.5碱性介质中阿仑膦酸钠经4-氯-7-硝基苯并-2-氧杂-1,3-二唑(NBF-Cl)衍生化后生成一种黄色荧光物质,该荧光物质经激发光照射产生荧光,据此提出了荧光光谱法测定中阿仑膦酸钠含量的方法。在激发波长457nm、发射波长532nm处,反应体系荧光强度与阿仑膦酸钠的质量浓度在0.02～1.0mg.L-1范围内呈线性关系。方法用于阿仑膦酸钠片剂的分析,测得加标回收率为97.1%～113%,相对标准偏差(n=6)为0.06%～0.14%。Based on the derivation reaction between sodium alendronate and 4-chloro-7-nitrobenzofurazan （NBF-CI） to give a yellow fluorescent product in a medium of pH 8. 5 and on the fluorescence property of the derivate as shown by its fluorescence spectrum at the wavelengths of 457 nm （λex） and 532 nm （λem）, a fluorophotometric method for determination of sodium alendronate was proposed. Linear relationship was found between values of fluorescence intensity and mass concentration of sodium alendronate in the range of 0. 02- 1.0 mg ·L^-1. The proposed method was used in the analysis of samples of sodium alendronate tablets, giving values of recovery ranged from 97. 1%-113%, and RSD＇s （n=6） ranged from 0. 06%-0. 14%.

关 键 词：荧光光谱法 阿仑膦酸钠 NBF-Cl 衍生反应 

分 类 号：O657.31[理学—分析化学]