体外染氡对V79细胞凋亡影响及其机制研究  

Effects and mechanisms of radon exposure on apoptosis of V79 cells in vitro

作　　者：韦晔[1] 刘玉萍[1] 吴周伟[1] 吕雪[2] 朱正保[1] 童建[1] 李建祥[1] 

机构地区：[1]苏州大学医学部公共卫生学院,江苏苏州215123 [2]苏州工业园区疾病防治中心,江苏苏州215123

出　　处：《扬州大学学报（农业与生命科学版）》2012年第3期13-16,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(81172707);江苏省自然科学基金资助项目(BK2012618)

摘　　要：建立中国仓鼠肺细胞(V79细胞)体外染氡早期损伤模型,对V79细胞凋亡的分子机制进行探析。筛选确定合适的染氡浓度及时间后将V79细胞分为对照组和染氡组,以每孔1.5×105个细胞接种于Transwell培养皿,细胞贴壁后将Transwell培养皿置于细胞动式气体染氡装置中染毒,染氡后收集各代细胞,常规培养后检测细胞周期、细胞凋亡及Caspase-3蛋白表达情况。结果表明:确定染氡浓度为40 000Bq·m-3,时间为10min,染毒后细胞G1期缩短,S期延长,细胞凋亡率逐渐升高,Caspase-3蛋白的表达有明显上升趋势,成功构建了V79细胞体外染氡早期损伤模型。在染毒早期阶段,V79细胞凋亡率上升与Caspase-3蛋白表达增加间无良好相关性。To establish an early radon exposure injury model of V79 ceils in vitro and investigate the mechanisms of apoptosis, V79 cells were divided into two groups, the control group （C） and the radon exposure group （Rn） after time and concentration of radon exposure were determined. 1.5 × 10^5 cells were planted onto Transwell membrane until getting adhered, then directly exposed to radon pumped in a gas inhalation box. After exposure cells were trypsinized into dishes for further growth. Cell cycle, apoptotic rate and expression of caspase-3 protein were detected through Flow Cytometeter and ELIASA. After 40 000 Bq · m-3 radon exposure for 10 min, G1 phase was significantly shorter while S phase was longer. The apoptotic rate and expression of Caspase-3 protein increased during radon exposure. V79 cells were built as an early radiation injury cell model successfully. During the early period of radon exposure, increased apoptotic rate of V79 cells was not correlated with increased Caspase-B protein expression.

关 键 词：肺癌 氡 中国仓鼠肺细胞 凋亡 CASPASE-3蛋白 

分 类 号：R114[医药卫生—卫生毒理学]