高温对液体悬浮培养发状念珠蓝细菌生长及生理生化的影响  

Response of Nostoc Flagelliforme in Liquid Suspension Culture to High Temperature Stress

作　　者：丁振[1,2] 贾士儒[1] 袁南南[1] 谭宁[1] 

机构地区：[1]天津科技大学工业发酵微生物教育部重点实验室,天津300457 [2]日照职业技术学院食品工程学院,山东日照276826

出　　处：《食品研究与开发》2012年第10期134-137,共4页Food Research and Development

基　　金：国家自然科学基金(20776112);国家自然科学基金(20376061)

摘　　要：研究高温对液体悬浮发状念珠蓝细菌细胞生长及生理生化的影响,将活化后菌种分别于30℃、35℃和40℃下培养,以25℃培养作为对照,测定细胞生长速率、胞外多糖和脯氨酸含量及SOD活性的变化。结果表明随着培养温度升高,细胞生长速率明显下降,SOD活性呈现先升高后降低的趋势;30℃和35℃培养条件下,胞外多糖和脯氨酸含量随培养温度的升高而增加,在40℃培养条件下,胞外多糖和脯氨酸含量先升高后下降。高温条件下,SOD为液体悬浮发状念珠蓝细菌提供快速的应激保护,而脯氨酸和胞外多糖为细胞提供较缓慢但更为持久的保护。Nostoc Flagelliforme is a cyanobacterium with high stress resistance.This experiment was conducted in order to study the growth conditions of Nostoc Flagelliforme when it was above the optimum growth temperature;and the resulting substantial changing law of it to meet the high temperature.Free-living cells were cultivated for 7 days at 25 ℃,30 ℃,35 ℃ and 40 ℃ separated in vitro.With different temperatures there were significant differences in cell growth.With the increase of the temperature,the activity of superoxide dismutase（SOD） declined after an obvious increase.At 30 ℃ and 35 ℃,the contents of proline（Pro） and extracellular polysaccharides（EPS） increased with a rise of temperature.But at 40 ℃,the content was increased,and then decreased.These results suggest that cell activity were apparently changed under the high temperature and SOD give N.flagelliforme a quick protection while the EPS,Pro give it a permanent one.

关 键 词：发状念珠蓝细菌 高温 液体悬浮培养 生理生化 

分 类 号：S647[农业科学—蔬菜学]