微量元素硒体外抗单纯疱疹病毒1型活性的初步研究  

Anti-herpes Simplex Virus Type 1 Activity of Trace Element Selenium in Vitro

作　　者：王安平[1] 余克花[2] 邹伟文[2] 宋矿余[2] 

机构地区：[1]南昌大学科学技术处,南昌330006 [2]南昌大学医学院微生物学教研室,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第9期1-4,共4页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫生厅科技计划(20053199);江西省科技厅社会发展科技攻关项目(20061A0402000)

摘　　要：目的初步研究微量元素硒体外抗单纯疱疹病毒1型(HSV-1)的活性及可能的机制。方法以四甲基偶氮唑盐(MTT)比色法测定硒化合物SeO2的最终作用的质量浓度和采用终点滴定法测定HSV-1的感染滴度,并通过观察细胞病变效应和细胞凋亡情况初步研究硒化合物SeO2体外抗HSV-1的活性。结果当SeO2药物质量浓度为0.382mg.L-1时,SeO2能明显地抑制病毒感染Vero细胞病变(CPE)以及促进细胞凋亡,其作用具有浓度依赖性;当SeO2药物质量浓度超过2.034mg.L-1时,细胞存活率下降。结论微量元素硒具有体外抗HSV-1的活性,且可通过抑制细胞病变和促进细胞凋亡而发挥作用。Objective To preliminarily investigate the anti-herpes simplex virus type 1 （HSV- 1）activity and possible action mechanism of trace element selenium in vitro. Methods The final reaction concentration of selenium compound （selenium oxide, SeOz ） was determined by MTT as- say, and the infectious titer of HSV-1 was detected by end-point titration. Anti-HSV-1 activity of SeOz was measured by cytopathic effect assay and apoptosis assay. Results The SeO2 （0. ：382 mg ·L^-1） obviously inhibited cytopathic effect and promoted apoptosis in a concentra- tion-dependent manner in Vero cells. However, the survival rates of cells significantly decreased at a SeO2 concentration higher than 2. 034 mg · L-1. Conclusion The trace element selenium can protect against HSV-1 challenge in vitro by inhibiting cytopathic effect and promoting cell apop- tosis.

关 键 词：单纯疱疹病毒1型 硒 抗病毒 体外 

分 类 号：R373.111[医药卫生—病原生物学] Q935[医药卫生—基础医学]