周围神经挤压伤后转化生长因子β的表达  

Expression of Transforming Growth Factor Beta in Peripheral Nerve Crushed Injury

作　　者：王德春[1] 陈中伟[1] 陈峥嵘[1] 张乃娴 宋后燕 

机构地区：[1]上海医科大学中山医院骨科

出　　处：《上海医科大学学报》2000年第2期121-123,共3页Journal of Fudan University(Medical Science)

基　　金：卫生部直属医疗机构临床学科重点项目!基金 ( 970 3 0 2 19)资助

摘　　要：目的　观察大鼠坐骨神经挤压伤后转化生长因子 β(TGF β)蛋白表达的变化 ,了解TGF β在周围神经损伤修复过程中所起的作用。方法　取 2 1只Wistar大鼠 ,造成坐骨神经挤压伤 ,伤后 6h、1、3、7、14、2 1d取材分别进行组织学、免疫组化观察。结果　 3d时神经挤压伤处TGF β抗原呈阳性反应 ,7d时损伤处的雪旺细胞明显增多 ,且TGF β表达的量也增多 ,以后TGF β表达的量有所减少。 结论　TGF β参与周围神经的损伤病理过程。Purpose To observe the changes of transforming growth factor beta (TGF?β) expression after rat sciatic nerve crushed injury and learning about the function of TGF?β in peripheral nerve injury and repair Methods Twenty one Wister rats were used.The sciatic nerves were curshed with a special clamp.The histology and immunohistochemistry examination were performed respectively after 6 hours,1,3,7,14,21 days injury. Results The TGF?β antibody in crushed sciatic nerve was positive after 3 days injury.The Schwann cells increased greatly and the TGF?β expression enhanced after 7 days injury.From then on,the expression of TGF?β decreased gradually. Conclusions The TGF?β participate in the pathoiogic process of peripheral nerve damage.

关 键 词：周围神经挤压伤 转化生长因子Β 坐骨神经 

分 类 号：R651.302[医药卫生—外科学]