十大功劳属部分植物茎中生物碱的高效毛细管电泳法测定  

DETERMINATION OF ALKALOIDS IN RHIZOMA OF SOME MAHONIA PLANTS BY HPCE

作　　者：纪秀红[1] 李奕[2] 刘虎威[2] 阎玉凝[1] 李家实[1] 

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京大学化学与分子工程学院,北京100871

出　　处：《药学学报》2000年第3期220-223,共4页Acta Pharmaceutica Sinica

摘　　要：目的 :对十大功劳属植物中 3种生物碱的含量进行测定 ,为其内在质量的评价提供科学依据。方法 :运用毛细管区带电泳 (CZE)模式 ,以马钱子碱为内标化合物 ,测定了小檗碱、巴马亭和药根碱的含量。背景缓冲溶液为0 1mol·L-1磷酸缓冲液 (pH 7 0 )与甲醇 ( 2∶1)的混合体系。线性范围 :盐酸小檗碱为 4 9～ 4 98 6μg·mL-1(γ =0 9990 ) ;盐酸巴马亭为 5 0～ 5 0 4 9μg·mL-1(γ =0 9996) ;盐酸药根碱为 5 1～ 5 0 5 8μg·mL-1(γ =0 9984 )。回收率 :盐酸小檗碱为 96 0 0 %～ 10 1 66% ;盐酸巴马亭为 10 0 15 %～ 10 2 97% ;盐酸药根碱为 96 68%～ 10 2 4 4%。结果 :不同品种、不同产地、不同采集时间的植物中的生物碱含量均有较大差别。结论 :高效毛细管电泳法是中草药质量控制的一种简便、快速、有效的方法。十大功劳属多种植物生物碱含量较高 。AIM: To determine the contents of three alkaloids in some plants of Mahonia and to provide an effective tool for the quality evaluation of Mahonia family. METHODS: The contents of three alkaloids, berberine, palmatine and jatrorrhizine, in different plants were determined by using high performance capillary electrophoresis (HPCE), with brucine as internal standard. The conditions were as follows: diode array detector set at 265 nm; run time, 8 0 min; applied voltage, 30 kV; temperature, 20℃. The background electrolyte system selected was 0 1 mol·L -1 phosphate with pH 7 0 methanol (2∶1). RESULTS: The linear ranges of berberine, palmatine, and jatrorrhizine were 4 9～498 6 μg·mL -1 (γ=0 9990), 5 0～ 504 9 μg·mL -1 (γ=0 9996) and 5 1～505 8 μg·mL -1 (γ=0 9984), respectively. The relative standard deviations of peak area of the alkaloids ranged from 1 03% to 1 61%. The average recoveries of the alkaloids were 96 00%～101 66%, 100 15%～102 97% and 96 68%～102 44%, respectively. Quantitative determination of the alkaloids in the dried stem of different plants of Mahonia was performed by this method. The results showed that the contents of the three alkaloids varied greatly with species, place of origin and collecting time. CONCLUSION: The contents of the alkaloids in all the plants of Mahonia analyzed in this paper were very high, so they have the potential of being new pharmaceutical resources. HPCE technique provides a simple, rapid, and effective tool for the quality control of herbal medicine.

关 键 词：十大功劳 毛细管电泳法 小檗碱 巴马亭 药根碱 

分 类 号：R284.1[医药卫生—中药学]