大鼠脊髓背角浅层神经元中神经肽YY_1受体与生长抑素共存  

Colocalization of neuropeptide Y Y_1 receptors with somatostatin in the neurons in superficial dorsal horn of rat spinal cord

作　　者：林英华[1] 朱俊玲[1] 童永光[1] 鲍岚[1] 

机构地区：[1]第四军医大学中国人民解放军神经科学研究所,西安710032

出　　处：《中国神经科学杂志》2000年第1期9-12,共4页

基　　金：国家自然科学基金!资助项目 ( 3 95 2 5 0 10 ;3 983 0 160 )

摘　　要：用间接免疫荧光双标记方法 ,研究大鼠脊髓 层神经元中神经肽 Y Y1受体与生长抑素共存关系。结果显示在 层深部许多神经元含 Y1受体 -免疫反应性物质 ,只观察到少量的生长抑素免疫反应阳性神经元胞体 ,其中个别 Y1受体阳性神经元也含生长抑素免疫反应性物质 ;在局部使用秋水仙素后 ,大多数 Y1受体免疫反应阳性神经元含有生长抑素免疫反应性物质。推论脊髓背角 层神经元中的 Y1受体可能与生长抑素在脊髓水平的功能有关。By using the indirect immunofluorescence method, the relationship between neuropeptide Y Y 1 receptor like immunoreactivity (LI) and somatostatin was studied in lamina Ⅱ of rat spinal cord. Y 1 receptor LI was expressed in many neurons in deeper lamina Ⅱ. However, only a small number of neuronal cell bodies were somatostatin positive. A few of Y 1 receptor positive also contained somatostatin LI. After local treatment of colchicine, most Y 1 receptor immunoreactive neurons in lamina Ⅱ contained somatostatin LI. This result suggests that Y 1 receptor in neurons of spinal dorsal horn may be correlated with the function of somatostatin at spinal level.

关 键 词：神经肽Y 生长抑素 脊髓 大鼠 

分 类 号：R338[医药卫生—人体生理学]