新疆地区汉族、维吾尔族先天性巨结肠患儿组织蛋白酶D与S-100的表达及其意义  

Expressions of cathepsin D and Soluble protein 100 and their clinicopathological significance in Hirschsprung′s disease in Hans and Uygurs in Xinjiang

作　　者：赵淑君 李学雄 査戈 祖木热提 曾岚 

机构地区：[1]乌鲁木齐市第一人民医院病理科,乌鲁木齐830011 [2]乌鲁木齐市第一人民医院儿外科,乌鲁木齐830011

出　　处：《新疆医科大学学报》2012年第10期1348-1351,1355,共5页Journal of Xinjiang Medical University

基　　金：乌鲁木齐市卫生局科技计划项目(200907)

摘　　要：目的探讨维吾尔族、汉族先天性巨结肠(hirschsprung’s disease,HD)患儿组织蛋白酶D(CAD)与S-100蛋白(S-100)表达及其临床意义。方法采用免疫组织化学法检测汉族、维吾尔族先天性巨结肠患儿CAD、S-100的水平,并与近端切缘的正常肠壁冰冻剩余组织进行比较。结果 (1)在HD扩张段和近端切缘的正常肠壁冰冻剩余组织的肠壁肌间和黏膜下层神经丛中神经节细胞CAD呈阳性表达,在神经纤维与神经鞘膜细胞均不表达。S-100在神经纤维与神经鞘膜细胞均阳性表达,神经节细胞阴性表达,表现为深染的神经丛中有细胞状"空白区"。(2)在HD狭窄段组肠壁神经丛中缺乏CAD阳性表达。S-100染色神经丛中未观察到细胞状"空白区";S-100强阳性表达的神经纤维明显增生,扭曲呈波浪状。(3)汉族先天性巨结肠患儿CAD、S-100与维吾尔族先天性巨结肠患儿CAD、S-100表达比较差异均无统计学意义(P>0.05)。结论 (1)CAD和S-100的免疫组化染色对诊断HD具有重要意义;(2)不同民族先天性巨结肠患儿CAD、S-100的表达均无明显差异;(3)CAD、S-100的检测简便、快速、经济,容易普及。Objective To investigate the expressions of cathepsin D（CAD） and S-100 protein（S100） and their significances in the colon of the patients with Hirschsprung′s disease（HD） in Hans and Uygurs.Methods Intestinal biopsy of 25 Hans and 20 Uygur cases with HD was made with SP immunohistochemical staining method,the levels of CAD and S-100 were tested,and then compared with the normal colon of frozen section remaining organization of proximal cut margin.Results（1） CAD was only positive in ganglionic cells in the expanding segments and the normal colon of frozen section remaining organization of proximal cut margin,which was observed in myenteric and submucosal plexuses,but in perineural satellite cells and nerve sheath cells,CAD was not expressed.Expression of S100 was contrary to CAD.The experession of S100 was positive in perineural satellite cells and nerve fibers and negative,in ganglionic cells,which left ＂cells-like blank area＂ in the plexuses.（2） In the stenotic segments of HD,neither CAD-positive neurons nor ＂cell-like blank area＂ in S100 staining were found in the plexus.The S100 positive fibers were proliferated obviously in number.There were obvious differences in the density of the S100 stained nerve fibers and the fiber sizes between the expanding and the stenotic segments of HD.（3） Expression of CAD and S100 had no statistic difference between the colon of the patients with HD in Hans and Uygurs（P0.05）.Conclusion（1）Detection CAD combined with S100 is a significant way in the diagnosis of HD.（2） The expression of CAD and S100 have no significant difference in the colon of the patients with HD in Hans and Uygurs.（3） It is economic and available to diagnose HD by detecting CAD and S100 with immunohistochemistry.

关 键 词：先天性巨结肠 汉族 维吾尔族 组织蛋白酶D(CAD) S-100蛋白 

分 类 号：R726.5[医药卫生—儿科] R392.11[医药卫生—临床医学]