斑点杂交法检测肝病患者TTV感染状况初探  

Detection of TTV DNA in sera of patients with non A-E hepatitis by dot blot hybridization

作　　者：张俊[1] 赵西平[1] 王宝菊 杨燕 李方和[1] 杨东亮[1] 

机构地区：[1]武汉同济医科大学同济医院临床免疫研究室,430030

出　　处：《肝脏》2000年第1期14-15,共2页Chinese Hepatology

摘　　要：目的　探讨斑点杂交法在检测肝病患者TT病毒(TTV)感染状况中的应用价值。方法　提抽患者血清中DNA,分别采用聚合酶链反应(PCR)及斑点杂交技术检测其中的TTV病毒DNA。结果　在30份非甲～非戊型肝炎患者血清中,PCR方法检出11份为TTV阳性,检出率为36.7%。采用斑点杂交法检出3份阳性血清,阳性率为10%。制备的探针具有TTV特异性,可检出相当于106拷贝的病毒DNA。结论　以地高辛标记的TTV探针用于斑点杂交可以检出血清病毒滴度较高的TTV,其敏感度低于PCR方法。Objective To explore the possibility of using dot blot hybridization in the detection of TTV virus infection.Methods Nuclear acid was extracted from serum specimens of patients with non A～E hepatitis, PCR and dot blot hybridization were carried out respectively to detect TTV DNA.Results Of 30 sera specimens, 11 were found positive by PCR, 3 were found positive by dot blot hybridization. The probe prepared was demonstrated to be TTV specific, and the sensitivity was determined to be capable of detecting 10 6 copies of viral DNA. Conclusion Dot blot hybridization with DIG labelled TTV DNA probe can be employed to detect TTV DNA in sera samples containing relatively high titer virus, with a sensitivity lower than that of PCR.

关 键 词：TTV病毒 聚合酶链反应 斑点杂交 肝炎 

分 类 号：R512.6[医药卫生—内科学]