22株耐亚胺培南肠杆菌科细菌KPC酶及整合子分布  

Distribution of KPC and Integrons in 22 Imipenem-resistant Enterobacteriaceae

作　　者：陶月 宁明哲 张葵 张之烽 

机构地区：[1]南京市鼓楼医院检验科,210008

出　　处：《医学研究杂志》2012年第10期95-98,共4页Journal of Medical Research

基　　金：南京市卫生局资助项目(YKK09115)

摘　　要：目的了解耐亚胺培南的肠杆菌科细菌KPC酶(klebsiella pneumoniae carbapenemase)及整合子分布情况。方法收集南京市鼓楼医院22株耐亚胺培南的肠杆菌科细菌,进行改良Hodge试验,PCR扩增检测细菌KPC酶与整合子基因并行序列分析。结果 22株肠杆菌科细菌中,改良Hodge试验阳性15株(68.2%),KPC酶扩增阳性15株(68.2%),整合子PCR扩增阳性6株(27.3%)。结论 KPC酶的流行需引起实验室及临床的关注。Objective To investigate the distribution of KPC and integrons in imipenem - resistant Enterobacteriaceae. Methods We modified Hodge test and PCR amplification for 22 imipenem - resistant Enterobacteriaceae from Nanjing Drum Tower Hospital, detec- ted KPC and integron of bacterial and did sequence analysis. Results In 22 Enterobacteriaeeae, 15 strains （68.2%） were positive in the modified Hodge, 15 strains （68.2%） were positive in KPC amplification, 6 strains （27.3%） were positive in PCR amplification of integron. Conclusion KPC Shold be paid attention as it may to cause tile prevalence of laboratory and clinical attention.

关 键 词：肠杆菌科细菌 KPC酶 整合子 

分 类 号：R378.2[医药卫生—病原生物学]