pH和氮素形态对紫花苜蓿根瘤特性的影响  

Effect of pH value and nitrogen forms on root nodule characteristics of alfalfa

作　　者：俞艳[1] 刘晓静[1] 齐敏兴[1] 张晓磊[1] 付萍[1] 王巧玲[1] 张晓玲[1] 李鸿燕[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2012年第5期7-11,共5页Grassland and Turf

基　　金：甘肃省自然基金项目"甘肃省秦王川灌区苜蓿草地施肥制度及其对环境的影响研究"(1010RJZA157);草业生态系统教育部重点实验室(甘肃农业大学)项目"供氮水平及氮素形态对紫花苜蓿养分吸收及根瘤特性的影响研究"(CYZS-2011012);甘肃农业大学本科生科研训练计划项目(SRTP)资助

摘　　要：采用砂培方法,在营养液条件下研究了pH和不同氮素形态对接种中华根瘤菌的甘农3号紫花苜蓿根瘤特性的影响。结果表明,苜蓿对碱性条件的耐受性强于酸性条件。过低或过高的pH对苜蓿与根瘤菌的共生固氮效果具有抑制作用;同时,铵态氮下培养的紫花苜蓿根瘤数、根瘤重、固氮酶活性、根系全氮含量和生物量均高于硝态氮下培养。表明pH接近于中性和铵态氮条件更有利于紫花苜蓿的结瘤固氮和植株的正常生长。To study the interaction of pH value and different nitrogen forms to root nodule characteristics of ＂GAU-III＂ alfalfa under the inoculation of rhizobia,alfalfa was grown in sand nutrient culture.The results showed that when pH value reached 7 or little higher,alfalfa grew well.And the tolerance in alkaline conndition of alfalfa was better than in acidic condition.Too low or too high pH value,the effect of symbiotic nitrogen-fixing of alfalfa and rhizobia would restrain.Meanwhile,root nodule number,nodulation,nitrogenaseactivity,root total nitrogen content,aboveground and underground biomass of alfalfa in ammonium nitrogen were higher than in nitrate nitrogen.It indicated that the condition of pH value close to neutral and ammonium nitrogen were more conducive to root nitrogen-fixing and regular growing of alfalfa.

关 键 词：PH 氮素形态 紫花苜蓿 结瘤固氮 

分 类 号：S144.5[农业科学—肥料学]