再生障碍性贫血患者外周血调节性T细胞、γδT细胞表达分析  

Analysis of Regulatory T Cells and γδT Cells in Patients with Aplastic Anemia(AA)

作　　者：刘永林[1] 周郁鸿[2] 陈益民[2] 谈潘莉[2] 陈婷婷[2] 朱琳超[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]浙江中医药大学附属第一医院

出　　处：《浙江中医药大学学报》2012年第10期1077-1078,1081,共3页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中医药重大疾病科技创新平台科研专项(2009ZDJB01);浙江省中医药科技计划项目(2011ZB049)~~

摘　　要：[目的]探讨再生障碍性贫血(aplastic anemia,AA)的免疫学发病机制。[方法]用流式细胞术检测32例急性再生障碍性贫血(acute aplastic anemia,AAA)、30例慢性再生障碍性贫血(chronic aplasitc anemia,CAA)患者、32例对照人群外周血淋巴细胞亚群、调节性T细胞、γδT细胞的表达。[结果]与对照组相比AAA、CAA组CD3+CD4+T细胞、CD4/CD8、调节性T细胞显著低于对照组,CD3+CD8+T细胞、γδT细胞显著高于对照组;AAA组与CAA组相比,AAA组γδT细胞高于CAA组,差异有统计学意义(P<0.05)。[结论]T淋巴细胞比例失调、调节性T、γδT细胞功能紊乱可能在AA发生、发展过程中发挥重要作用。[Objective] To discuss the roles of the pathogenesis of AA.[Methods] The expressions of lymphocyte subsets,regulatory T cells and γδT cells in peripheral blood in 32 patients with AAA and 30 patients with CAA were detected by flow cytometry（FCM）,and compared with 32 persons with normal control.[Results]The percentages of peripheral blood CD3＋CD4＋T cells,CD4/CD8 and regulatory T cells in the groups of AAA and CAA were significantly lower than those of control group.There were significant differences of the ratio of CD3＋CD8＋T cells,γδT cells in peripheral blood between AAA,CAA groups and the control group（P0.05）,and in comparison with AAA and CAA,the percentage of γδT cells in AAA was significantly higher than CAA.[Conclusion] The ratio imbalance of T lymphocyte and dysfunction of regulatory T cells and γδT cells might play a major role in the pathogenesis of AA.

关 键 词：AA T细胞亚群 B细胞 NK细胞 调节性T细胞 ΓΔT细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]