HPLC法测定通光藤药材及消癌平片中通光藤苷A  

Determination of tenacissosides A in Marsdeniae Tenacissimae Caulis and Xiaoaiping Tablets by HPLC method

作　　者：陈敏[1] 李媛媛[2] 李先荣[1] 

机构地区：[1]山西省中医药研究院方剂研究所,山西太原030012 [2]北京中医药大学,北京100102

出　　处：《药物评价研究》2012年第5期362-364,共3页Drug Evaluation Research

基　　金：通关藤种质鉴定与药材质量相关性研究(2011011035-1)

摘　　要：目的建立通光藤药材及消癌平片中通光藤苷A的HPLC测定方法。方法色谱柱为Sinochrom ODS-BP分析柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(41:59);检测波长230nm。结果通光藤苷A在20.2～404.0μg与峰面积积分值呈良好的线性关系,r=0.99995,平均加样回收率为96.6%,RSD值为2.19%(n=6)。结论该方法简单,结果准确,稳定性好,重现性高,可用于通光藤药材及消癌平片定量测定及质量控制。Objective To develop an HPLC method for determination of tenacissosides A in Marsdenia tenacissima and Xiaoaiping Tablets.Methods Sinochrom ODS-BP column was used（250 mm × 4.6 mm,5 μm）,the mobile phase consisted of acetonitrile-0.1%（41：59）,and the UV detection wavelength was at 230 nm.Results Good linear correlation for tenacissosides A was found in the concentration range of 20.2―404.0 μg（r = 0.999 95）.The average recovery rate was 96.6%,with RSD of 2.19%（n = 6）.Conclusion The method is simple,accurate,and stable which is fit for determination and quality control of Marsdeniae Tenacissimae Caulis and Xiaoaiping Tablets.

关 键 词：通光藤 消癌平 通光藤苷A HPLC 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]