RP-HPLC法测定生津益气口服液中黄芪甲苷的含量  

Determination of astragaloside IV in Shengjinyiqi Oral Liquid by RP-HPLC

作　　者：刁保忠[1] 靳维荣[1] 王登旭 石磊[1] 

机构地区：[1]聊城市人民医院,山东聊城252002 [2]聊城市药品检验所,山东聊城252000

出　　处：《齐鲁药事》2012年第10期588-589,共2页qilu pharmaceutical affairs

摘　　要：目的建立高效液相色谱法测定生津益气口服液中黄芪甲苷的含量。方法采用高效液相色谱法,蒸发光散射检测器,色谱柱为Agilent C18柱,流动相为乙腈-水(32∶68),流速为1.0 mL.min-1,进样量为10μL,柱温为35℃,ELSD检测器漂移管温度为100℃,载气流量为2.7 L.min-1。结果黄芪甲苷在0.11～0.90 mg.mL-1范围内呈良好的线性关系,r=0.999 3。平均加样回收率为97.3%,RSD为1.13%(n=6)。结论该法简便,准确,专属性强,可用于生津益气口服液的质量控制。Objective To establish an HPLC method for the determination of astragaloside IV in Shengjinyiqi Oral Liquid. Methods HPLC -ELSD was adopted. The column was Agilent C18 column,the mobile phase was acetonitrile -water （32： 68） and the flow rate was 1.0 mL. min^-1. The injection volume was 10 μL. The column temperature was 35 ℃. The drift tube temperature of ELSD detector was 100 ℃ and the flow rate of the carrier gas was 2.7 L . min^ -1. Results Astragaloside IV showed a good linear relationship within the range of 0.11 -0.90 mg .mL^- 1 , r = 0. 999 3. The average recovery was 97.3 % and the RSD was 1. 13% （ n = 6）. Conclusion The method was simple,accurate and specific,and it can be used for the quality control of the Shengjinyiqi Oral Liquid.

关 键 词：生津益气口服液 黄芪甲苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]