枸骨叶中熊果酸的提取工艺研究  

STUDY ON EXTRACTION PROCESS OF URSOLIC ACID IN CORNUTA LEAF

作　　者：李开泉[1,2] 曾晓敏[3] 

机构地区：[1]江西省天然药物活性成分研究重点实验室,江西宜春336000 [2]宜春学院化学与生物工程学院,江西宜春336000 [3]宜春学院教育科技园,江西宜春336000

出　　处：《井冈山大学学报（自然科学版）》2012年第6期21-23,共3页Journal of Jinggangshan University (Natural Science)

基　　金：国家"863"高技术研究发展计划项目[2002AA2Z3217]

摘　　要：研究枸骨叶中熊果酸的最佳提取工艺条件。在单因素试验的基础上,采用正交试验设计法,选择乙醇浓度（A）、固液比（B）、提取时间（C）为因素,每因素各取三水平,按L_9（3~4）正交表进行实验。结果表明：影响提取效果各因素为乙醇浓度〉提取时间〉固液比。其最优提取工艺条件为用8.5倍量φ=95%乙醇,于80℃加热回流提取2次,第一次2h,第二次1.5h。To study the best extraction process of ursolic acid in leaves of Ilex cornuta.Based on the single-factor test,experiment was carried out according to L9（34） orthogonal table by orthogonal experimental design method,selecting the ethanol concentration（A）,solid-liquid ratio（B）,extraction time（C） as factors,three levels for each factor.The results showed that the factors affecting the extraction were ethanol concentration extraction time solid-liquid ratio.The optimum extraction conditions were that heating reflux extraction were done for two times using 8.5 times the amount of φ = 95% ethanol at 80 ℃,2 h for the first,1.5 h for the second.

关 键 词：枸骨叶 熊果酸 提取工艺 正交实验 

分 类 号：S567.19[农业科学—中草药栽培]