花菖蒲类鸢尾组培愈伤组织诱导及芽的分化研究  

The Callus Induction and Shoot Differentiation of Pedicels in Tissue Culture of Iris ensata L.

作　　者：王文元[1] 王文和[2] 周文强[1] 王丹[1] 史国旭[3] 许玉凤[3] 

机构地区：[1]沈阳市植物园,辽宁沈阳110163 [2]北京农学院,北京102206 [3]沈阳农业大学,辽宁沈阳110866

出　　处：《北方园艺》2012年第21期92-94,共3页Northern Horticulture

基　　金：沈阳市科技局农业科技攻关资助项目(F10-099-3-00)

摘　　要：以日本花菖蒲类鸢尾的2个品种为试材,进行了愈伤组织的诱导和不定芽分化的组织培养试验。结果表明:日本花菖蒲类鸢尾组织培养最佳的外植体是花茎,而且是在花苞长度为2～5cm左右处取样最佳,灭菌以采用0.1%HgCl2消毒10min效果最好,污染率仅为15%,成活率能达75%。诱导愈伤组织和不定芽分化的最佳培养基为MS+6-BA 1.0mg/L+NAA 1.0mg/L,愈伤组织诱导率为59.09%,不定芽的分化率为50%。Two species of Japanese Iris ensata L.were used as test materials to develop callus induction and shoot differentiation of pedicels.The results showed that flower stalks was the best explants when the length of flower bud was 2~5 cm,and disinfected with 0.1% HgCl2 for 10 min were the most effective with the average contamination rate of 15.00% and survival rate of 75%.The optimal medium developing callus induction and shoot differentiation of pedicels was MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L NAA,the induction rate was 59.09% and differentiation rate was 50%.

关 键 词：日本花菖蒲 组织培养 诱导 分化 

分 类 号：S682.24[农业科学—观赏园艺]