无籽西瓜“蜜童”子叶再生体系的建立  

Establishment of Cotyledon Explants Regeneration System in Seedless Watermelon 'Mitong'

作　　者：张娜[1,2] 彭定祥[2] 孙玉宏 曾红霞 施先锋 任俭 蒋卉[2] 安霞[2] 

机构地区：[1]武汉市农业科学研究所,湖北武汉430345 [2]华中农业大学植物科学技术学院,湖北武汉430070

出　　处：《北方园艺》2012年第21期100-102,共3页Northern Horticulture

基　　金：武汉市科技攻关资助项目(201021112428-9);武汉市农业科学技术研究院创新资助项目

摘　　要：以无籽西瓜"蜜童"无菌苗子叶为外植体,建立了高效的组培再生体系。结果表明:不定芽诱导培养基为MS+1.0mg/L 6-BA+0.1mg/L NAA,诱导率达88.33%,最佳伸长培养基为MS+0.2mg/L 6-BA,最佳生根培养基为1/2MS+0.5mg/L IBA,生根率达90%。Cotyledon excised from seedlings of watermelon ＇Hitong＇ were used as explants,an efficient plant regeneration system via organogenesis was established in triploid watermelon ＇Mitong＇.The results showed that 88.33% explants could regenerate to adventitious shoots on MS medium with 1.0 mg/L 6-BA and 0.1 mg/L NAA.The elongation media was MS＋0.2 mg/L 6-BA.90% adventitious shoots were successfully rooted on a 1/2MS medium with 0.5 mg/L IBA.

关 键 词：无籽西瓜 子叶 再生体系 

分 类 号：S651[农业科学—果树学]