根癌农杆菌介导IFS基因对岷山红三叶遗传转化研究  

Genetic Transformation Isoflavones Synthase Gene from Trifolium pratense Minshan Callus Mediated by Agrobacterium Tumefaciens

作　　者：胡焕焕[1] 李卫东[1] 姬国杰[1] 丰慧根[1] 

机构地区：[1]新乡医学院生命科学技术系,河南新乡453000

出　　处：《北方园艺》2012年第21期110-112,共3页Northern Horticulture

基　　金：河南省科技攻关资助项目(102102310189);河南省教育厅科技攻关资助项目(2009A180014);新乡医学院研究生科研创新资助项目(YJSCX201116Y)

摘　　要：以岷山红三叶草为试材,以其子叶和叶柄的愈伤组织为受体,采用根癌农村菌介导法,研究其遗传转化的条件,以期建立根癌农杆菌介导的异黄酮合酶IFS基因遗传转化体系。结果表明:利用该转化体系,获得的抗性愈伤组织,通过提取其基因组进行PCR扩增鉴定,证实为转化子。提示根癌农杆菌介导转化岷山红三叶愈伤组织是完全可行的,为利用基因工程方法改良红三叶的品种并获得高产异黄酮品系奠定了基础。The callus was induced from the cotyledons and petioles of Trifolium pratense Minshan,and the callus was used for the receptor of genetic transformation mediated by agrobacterium tumefaeiens.The resistant callus was screened and confirmed to be transformants by PCR.The results showed that genetic transformation of Trifolium pratense Minshan callus mediated by Agrobaetenum tumefaeiens was entirely feasible.

关 键 词：岷山红三叶 愈伤组织 根癌农杆菌 异黄酮合酶IFS 遗传转化 

分 类 号：S541.2[农业科学—作物学]