止消通脉宁对转化生长因子-β_1诱导的人肾小管上皮细胞Ⅰ、Ⅲ型胶原及纤连蛋白mRNA表达的影响  

Effect of Zhixiaotongmaining on Expression of ColⅠ,Col Ⅲ and FN mRNA of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β_1

作　　者：张邦能[1,2] 杨丽霞[1,2] 程涛[1,2] 刘铜华[3] 吴丽丽[3] Margetts Peter Joseph 

机构地区：[1]甘肃省中医院 [2]甘肃省中医药研究院,甘肃兰州730050 [3]北京中医药大学,北京100029 [4]加拿大麦克马斯特大学

出　　处：《中国中医药信息杂志》2012年第11期33-35,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：科技部国际科技合作项目(2009DFA31520)

摘　　要：目的探讨止消通脉宁含药血清对转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)和纤连蛋白(FN)mRNA表达的影响。方法将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养,分为空白对照组、TGF-β1诱导组(TGF-β110 ng/mL)、空白血清对照组(TGF-β110 ng/mL+10%空白血清)、干预1组(TGF-β110 ng/mL+10%低剂量止消通脉宁含药血清)、干预2组(TGF-β110 ng/mL+10%中剂量止消通脉宁含药血清)、干预3组(TGF-β110 ng/mL+10%高剂量止消通脉宁含药血清)。药物干预24 h后,荧光定量PCR检测ColⅠ、ColⅢ和FN的mRNA表达。结果 HK-2细胞经TGF-β1诱导后,ColⅠ、ColⅢ和FN的mRNA表达显著上升,与空白对照组比较,差异有统计学意义(P<0.05)。经止消通脉宁含药血清干预后,其表达逐步下降,与单纯TGF-β1诱导组比较,差异有统计学意义(P<0.05)。空白血清无此作用。结论止消通脉宁在一定程度上能够抑制TGF-β1诱导的人肾小管上皮细胞ColⅠ、ColⅢ和FN的mRNA表达,减少细胞外基质成分的分泌,具有防治肾间质纤维化作用。Objective To explore the effect of Zhixiaotongmaining（ZXTMN） on the expression of ColⅠ,Col Ⅲ and FN mRNA of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1（TGF-β1）.Methods The HK-2 cells were cultured by DMEM/F12（1∶1） with 10% fetal bovine serum and divided into control group,TGF-β1 group（TGF-β1 10 ng/mL）,rat serum control group（TGF-β1 10 ng/mL＋10% rat serum）,ZXTMN-containing rat serum therapy groups（TGF-β1 10 ng/mL＋10% low dose ZXTMN,or ＋10% medium dose ZXTMN,or ＋10% high dose ZXTMN）.After 24 h,the expression of ColⅠ,Col Ⅲ and FN mRNA were tested by fluorescence quantitaty PCR.Results The expression of ColⅠ,Col Ⅲ and FN mRNA of HK-2 cell cultured with TGF-β1 were much notable than the control,and significantly decreased in HK-2 cell cultured with TGF-β1 plus ZXTMN compared with only TGF-β1（P0.05）,but only rat serum had no effect.Conclusion ZXTMN can inhibit the expression of ColⅠ,Col Ⅲ and FN mRNA of HK-2 cell induced by TGF-β1,and can prevent the development of renal fibrosis to a certain extent.

关 键 词：止消通脉宁 转化生长因子-Β1 人肾小管上皮细胞 Ⅰ型胶原 Ⅲ型胶原 纤连蛋白 

分 类 号：R285.5[医药卫生—中药学]