骨刺消巴布剂对兔膝关节骨性关节炎软骨细胞凋亡的影响  

Effect of Gucixiao Cataplasm on Osteoarthritis Cartilage Cell Apoptosis in Rabbit Knee

作　　者：邢涛[1] 董林[1] 魏国俊[1] 雷宁波[1] 王志勇[1] 徐玉德[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050

出　　处：《中国中医药信息杂志》2012年第11期39-41,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省中医药科学技术研究课题(GZK-2011-66)

摘　　要：目的观察骨刺消巴布剂对兔膝关节骨性关节炎软骨细胞凋亡和凋亡调控基因bcl-2、bax的影响,并探讨其作用机理。方法选用健康日本大耳白兔40只,随机分为骨刺消巴布剂组、奇正消痛贴组、空白对照组、正常组。Videman固定法进行改良制备兔膝关节骨性关节炎模型,骨刺消巴布剂组、奇正消痛贴组外敷相应药物,空白对照组、正常组不做任何处理,给药5周后处死动物,TUNEL法检测软骨细胞凋亡,免疫组化法检测关节软骨中bcl-2、bax的水平。结果骨刺消巴布剂组、奇正消痛贴组细胞凋亡率明显低于空白对照组(P<0.05);bcl-2在骨刺消巴布剂组、奇正消痛贴组中的表达高于空白对照组(P<0.05),bax的表达低于空白对照组(P<0.05),骨刺消巴布剂组与奇正消痛贴组比较差异无统计学意义。结论骨刺消巴布剂可能是通过上调bcl-2的表达、下调bax的表达来抑制兔膝关节骨性关节炎软骨细胞的过度凋亡,这可能是该药治疗骨性关节炎的机制之一。Objective To observe the effect of Gucixiao cataplasm on osteoarthritis cartilage cell apoptosis,apoptosis gene bcl-2 and its inhibition gene bax in rabbit knee,and explore its mechanism.Methods Forty healthy Japanese rabbits were randomly divided into Gucixiao cataplasm group,Qizheng slake pain patch group,blank control group and the normal group.Gucixiao cataplasm group,Qizheng slake pain patch group and blank control group were prepared through the classic Videman fixed method to improve rabbit knee osteoarthritis models.Gucixiao cataplasm group and Qizheng slake pain patch group were applied with corresponding drugs,blank control group and the normal group group without any treatment for 5 weeks.After the death of animal,TUNEL method was used for the detection of cartilage cell apoptosis,and immunohistochemistry for the detection of particular cartilage bcl-2 and bax.Results Apoptosis rate of Gucixiao cataplasm group and Qizheng slake pain patch group was significant lower than blank control group（P0.05）,bcl-2 expression in Gucixiao cataplasm group and Qizheng slake pain patch group was higher than blank control group（P0.05）,bax expression was lower than blank control group（P0.05）.Gucixiao cataplasm group and Qizheng slake pain patch group had no significant statistical difference.Conclusion Gucixiao cataplasm can inhibit osteoarthritis cartilage cell of excessive apoptosis in rabbit knee through up-regulation of bcl-2 expression and down-regulation of bax expression.This is one of mechanism for treating osteoarthritis by Gucixiao cataplasm.

关 键 词：膝关节骨性关节炎 骨刺消巴布剂 细胞凋亡 BCL-2蛋白 BAX蛋白 兔 

分 类 号：R285.5[医药卫生—中药学]