高效液相色谱法测定不同种蟾皮中蟾蜍噻咛的含量  

Determination of Bufothionine in Different Species of Toad Skin by HPLC

作　　者：王宏洁[1] 杨立新[1] 高波 程明[1] 杨健[1] 边宝林[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]安徽金蟾生化药业有限公司,安徽淮北235000

出　　处：《中国中医药信息杂志》2012年第11期44-45,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金："重大新药创制"国家科技重大专项(2009ZX09103-404);中国中医科学院基本科研业务费自主选题项目(Z02063)

摘　　要：目的对不同种的蟾皮中蟾蜍噻咛的含量进行研究。方法采用高效液相色谱法测定不同种蟾皮中蟾蜍噻咛的含量,色谱柱WondsilTMC18(4.6 mm×250 mm,5μm),流动相乙腈-水(6∶94),检测波长226 nm,流速1.0 mL/min,柱温35℃。结果回归方程为Y=6 945.3X+82.327,r2=0.999 4,平均回收率为98.57%,蟾皮中蟾蜍噻咛的质量分数在0.041 2～0.824μg。结论蟾蜍噻咛在不同种蟾皮药材中的含量有一定差异。Objective To determine bufothionine in different species of dried toad skin. Methods HPLC analysis was performed on WondsilTM C18 column （4.6 mm×250 mm, 5 μm） with acetonitrile-water （6 ： 94） as mobile phase. The detection wavelength was 226 nm. Flow rate was 1.0 mL/min, and the column temperature was 35 ℃. Results The calibration curve was developed （Y =6 945.3X ＋82.327） with correlation efficient of 0.999 4. Bufothionine was detected within 0.041 2--0.824 μg in different toad skin with the average recovery rate of 98.57%. Conclusion There was a certain difference of bufothionine contents in different species of toad skin.

关 键 词：中华大蟾蜍 黑框蟾蜍 牛蛙 蟾蜍噻咛 蟾皮 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]