咽炎方含片高效液相色谱指纹图谱研究  

HPLC Fingerprint of Yanyanfang Buccal Tablet

作　　者：韦炳华[1] 任斌[1] 卢进[1] 李瑞明[1] 傅晓华[2] 陈孝[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080 [2]广州新海医院药剂科,广东广州510300

出　　处：《中国中医药信息杂志》2012年第11期49-51,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：广东省重大科技专项(2011A080300004)

摘　　要：目的建立咽炎方含片高效液相色谱(HPLC)指纹图谱。方法采用Phenomenex C18柱(250 mm×4.6 mm,5μm),流动相为0.2%磷酸溶液(A)-乙腈(B),梯度洗脱,检测波长203 nm,柱温为室温,流速1.0 mL/min。结果该色谱条件下咽炎方含片各成分得到了较好的分离,标准指纹图谱由9个特征峰组成。10个批次的样品指纹图谱相似度为0.858 6～0.990 2。结论 HPLC指纹图谱具有重复性好、特征性强、方法简便等特点,可用于咽炎方含片的质量控制。Objective To establish HPLC fingerprint of Yanyanfang Buccal Tablet.Methods Phenomenex C18 column（250 mm×4.6 mm,5 μm） was used with acetonitrile-0.2% phosphoric acid（gradient elution） as the mobile phase,detection wavelength was set at 203 nm,flow rate was 1 mL/min,and column temperature was ambient.Results The components of Yanyanfang Buual Tablet were well separated under the conditions.Nine common peaks were identified.The similarity among 10 batches of samples was 0.8586-0.9902.Conclusion The method is reliable,accurate and suitable for the quality control of Yanyanfang Buccal Tablet.

关 键 词：咽炎方含片 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]