参附注射液对实验性心力衰竭大鼠心肌细胞凋亡干预作用的研究  

Intervention study of Shenfu Injection on cardiomyocyte apoptosis in experimental heart failure rats

作　　者：郑思道 吴红金 余少平 多唯韦 任建勋 王升启[2] 刘宇娜 杨继媛 

机构地区：[1]北京市中西医结合医院,北京100039 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华中医药杂志》2012年第11期2972-2975,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市中医药科技项目(No.JJ2001-37);国家自然科学基金项目(No.81173367)~~

摘　　要：目的:研究参附注射液对心力衰竭大鼠心肌凋亡和凋亡相关蛋白的影响。方法:45只Wistar大鼠随机分为对照组、模型组和治疗组,采用左冠状动脉结扎法建立心力衰竭模型。对照组和模型组给予0.9%氯化钠溶液、参附组给予腹腔注射参附注射液(6.2mL.kg-1.d-1,4周)。采用TUNEL和免疫组化检测心肌凋亡和相关蛋白。结果:共有38只大鼠存活。对照组(n=14)未见心肌凋亡,模型组(n=13)凋亡数量增加,而参附组(n=11)凋亡数量明显减少,与模型组比较差异具有显著性(P<0.01)。与对照组相比,模型组Bax、Bcl-2、Fas和FasL蛋白表达增加(P<0.05);与模型组相比,治疗组Bax、Fas和FasL蛋白表达显著下降(P<0.01)。结论:参附注射液能够抑制实验性心力衰竭大鼠心肌凋亡,其作用机制与下调促凋亡基因Bax、Fas和FasL表达的作用相关。Objective： To investigate the intervention of Shenfu Injection on cardiomyocyte apoptosis and expressions of apoptosis-related proteins in heart failure rats.Methods： Forty-five Wistar rats were randomized into 3 groups： sham,heart failure model and Shenfu Injection.Heart failure was introduced by ligation of the left anterior descending coronary artery.38 rats survived,and animals in the sham group（n=14） and model group（n=13） received a saline injection while animals in the Shenfu Injection group（n=11） received an Injection of Shenfu Injection（6.2mL.kg-1.d-1 ip,4w）.Myocardial cells were measured by TdT-mediated dUTP nick end labeling（TUNEL）,and the expressions of Bax,Bcl-2,Fas and FasL in cardiomyocytes were detected by immunohistochemical assays.Results： The TUNEL stained cardiomyocytes significantly increased,and the expressions of Bcl-2,Fas,FasL as well as Bax in cardiomyocytes significantly up-regulated in model group compared with those in sham rats（P0.05）.Compared with the model group,it was in the Shenfu Injection group that expressions of Bax,Fas,FasL and TUNEL stained cardiomyocytes significantly down-regulated after 4-week Shenfu Injection therapy（P0.01）.Conclusion： Shenfu Injection could Inhibit cardiomyocyte apoptosis in experimental heart failure rats by down-regulating expressions of Bax,Fas and FasL.

关 键 词：参附注射液 心力衰竭 心肌细胞凋亡 Bax FAS FASL Bcl-2 

分 类 号：R285.5[医药卫生—中药学]